Elevated serum IgA associated with immunoproliferative enteropathy of Basenji dogs

Lack of evidence for alpha heavy-chain disease or enhanced intestinal IgA secretion

E. V. De Buysscher, E. B. Breitschwerdt, Nigel J Maclachlan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Immunoglobulin isotypes in serum and intestinal secretions of Basenji dogs with chronic diarrhea, asymptomatic Basenji dogs, and healthy control dogs were quantitated and their molecular sizes characterized to detect α-chain, γ-chain, or μ-chain fragments. Quantitation of immunoglobulin isotypes in serum showed that affected Basenjis have significantly elevated serum IgA values as compared to asymptomatic Basenjis and normal control dogs. However, IgA concentrations in intestinal wash fluids were not significantly different for the three groups. Immunoelectrophoresis (IEP) and polyacrylamide gel electrophoresis (PAGE) demonstrated that virtually all IgA was in the dimeric form. Using IEP and immunoselection, we were unable to detect evidence for the presence of α-chain or other heavy-(H)-chain fragments. Hyperimmune serum obtained from rabbits immunized with serum or a globulin fraction of affected Basenjis also failed to detect H-chain fragments. The results of this study indicate that immunoproliferative enteropathy of Basenjis resembles closely the nonsecretory form of human immunoproliferative small intestinal disease (IPSID).

Original languageEnglish (US)
Pages (from-to)41-52
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume20
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Immunoproliferative Small Intestinal Disease
Heavy Chain Disease
Intestinal Secretions
blood serum
digestive system diseases
Immunoglobulin A
Dogs
immunoelectrophoresis
dogs
Serum
Immunoelectrophoresis
immunoglobulins
Immunoglobulin Isotypes
globulins
polyacrylamide gel electrophoresis
Globulins
antiserum
diarrhea
rabbits
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

@article{2037d3e3f3ba4204a4bb2085d62a70c5,
title = "Elevated serum IgA associated with immunoproliferative enteropathy of Basenji dogs: Lack of evidence for alpha heavy-chain disease or enhanced intestinal IgA secretion",
abstract = "Immunoglobulin isotypes in serum and intestinal secretions of Basenji dogs with chronic diarrhea, asymptomatic Basenji dogs, and healthy control dogs were quantitated and their molecular sizes characterized to detect α-chain, γ-chain, or μ-chain fragments. Quantitation of immunoglobulin isotypes in serum showed that affected Basenjis have significantly elevated serum IgA values as compared to asymptomatic Basenjis and normal control dogs. However, IgA concentrations in intestinal wash fluids were not significantly different for the three groups. Immunoelectrophoresis (IEP) and polyacrylamide gel electrophoresis (PAGE) demonstrated that virtually all IgA was in the dimeric form. Using IEP and immunoselection, we were unable to detect evidence for the presence of α-chain or other heavy-(H)-chain fragments. Hyperimmune serum obtained from rabbits immunized with serum or a globulin fraction of affected Basenjis also failed to detect H-chain fragments. The results of this study indicate that immunoproliferative enteropathy of Basenjis resembles closely the nonsecretory form of human immunoproliferative small intestinal disease (IPSID).",
author = "{De Buysscher}, {E. V.} and Breitschwerdt, {E. B.} and Maclachlan, {Nigel J}",
year = "1988",
doi = "10.1016/0165-2427(88)90024-4",
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T2 - Lack of evidence for alpha heavy-chain disease or enhanced intestinal IgA secretion

AU - De Buysscher, E. V.

AU - Breitschwerdt, E. B.

AU - Maclachlan, Nigel J

PY - 1988

Y1 - 1988

N2 - Immunoglobulin isotypes in serum and intestinal secretions of Basenji dogs with chronic diarrhea, asymptomatic Basenji dogs, and healthy control dogs were quantitated and their molecular sizes characterized to detect α-chain, γ-chain, or μ-chain fragments. Quantitation of immunoglobulin isotypes in serum showed that affected Basenjis have significantly elevated serum IgA values as compared to asymptomatic Basenjis and normal control dogs. However, IgA concentrations in intestinal wash fluids were not significantly different for the three groups. Immunoelectrophoresis (IEP) and polyacrylamide gel electrophoresis (PAGE) demonstrated that virtually all IgA was in the dimeric form. Using IEP and immunoselection, we were unable to detect evidence for the presence of α-chain or other heavy-(H)-chain fragments. Hyperimmune serum obtained from rabbits immunized with serum or a globulin fraction of affected Basenjis also failed to detect H-chain fragments. The results of this study indicate that immunoproliferative enteropathy of Basenjis resembles closely the nonsecretory form of human immunoproliferative small intestinal disease (IPSID).

AB - Immunoglobulin isotypes in serum and intestinal secretions of Basenji dogs with chronic diarrhea, asymptomatic Basenji dogs, and healthy control dogs were quantitated and their molecular sizes characterized to detect α-chain, γ-chain, or μ-chain fragments. Quantitation of immunoglobulin isotypes in serum showed that affected Basenjis have significantly elevated serum IgA values as compared to asymptomatic Basenjis and normal control dogs. However, IgA concentrations in intestinal wash fluids were not significantly different for the three groups. Immunoelectrophoresis (IEP) and polyacrylamide gel electrophoresis (PAGE) demonstrated that virtually all IgA was in the dimeric form. Using IEP and immunoselection, we were unable to detect evidence for the presence of α-chain or other heavy-(H)-chain fragments. Hyperimmune serum obtained from rabbits immunized with serum or a globulin fraction of affected Basenjis also failed to detect H-chain fragments. The results of this study indicate that immunoproliferative enteropathy of Basenjis resembles closely the nonsecretory form of human immunoproliferative small intestinal disease (IPSID).

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