Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue

S. J. Shih, Marc Dall'Era, J. R. Westphal, J. Yang, C. G J Sweep, Regina F Gandour-Edwards, Christopher P Evans

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens. Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD. Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalProstate Cancer and Prostatic Diseases
Volume6
Issue number2
DOIs
StatePublished - 2003

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Prostatic Hyperplasia
Microvessels
Prostate
Angiostatins
Plasminogen Inactivators
Angiogenesis Inhibitors
Plasminogen Activator Inhibitor 1
Urokinase-Type Plasminogen Activator
Factor VIII
Fibroblast Growth Factor 2
Tissue Plasminogen Activator
Prostatectomy
Immunoassay
Immunoblotting
Vascular Endothelial Growth Factor A
Prostatic Neoplasms
Cytokines
Carcinoma
Antibodies
Proteins

Keywords

  • Angiostatin
  • Basic fibroblast growth factor
  • Microvessel density
  • Primary prostate culture
  • Urokinase
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

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title = "Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue",
abstract = "Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens. Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD. Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.",
keywords = "Angiostatin, Basic fibroblast growth factor, Microvessel density, Primary prostate culture, Urokinase, Vascular endothelial growth factor",
author = "Shih, {S. J.} and Marc Dall'Era and Westphal, {J. R.} and J. Yang and Sweep, {C. G J} and Gandour-Edwards, {Regina F} and Evans, {Christopher P}",
year = "2003",
doi = "10.1038/sj.pcan.4500637",
language = "English (US)",
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TY - JOUR

T1 - Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue

AU - Shih, S. J.

AU - Dall'Era, Marc

AU - Westphal, J. R.

AU - Yang, J.

AU - Sweep, C. G J

AU - Gandour-Edwards, Regina F

AU - Evans, Christopher P

PY - 2003

Y1 - 2003

N2 - Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens. Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD. Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.

AB - Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens. Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD. Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.

KW - Angiostatin

KW - Basic fibroblast growth factor

KW - Microvessel density

KW - Primary prostate culture

KW - Urokinase

KW - Vascular endothelial growth factor

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