Electrophysiologic and molecular properties of cultured enteric glia

D. L. Broussard, P. G C Bannerman, C. M. Tang, M. Hardy, David E Pleasure

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Enteric glia, the support cells of myenteric ganglia, have been widely studied with respect to their morphology and immunohistochemical phenotype, but little is known about their functional properties. We developed a method for the amplification of enteric glia from newborn guinea pigs to further characterize these cells. Treatment with a combination of basic fibroblast growth factor and the adenylate cyclase activator, cholera toxin, permitted expansion of enteric glial cultures to confluence and serial passage for up to 8 months. The long-term cultured cells retained expression of 1) S100 protein, 2) GD3 ganglioside recognized by the monoclonal antibody LB1, and 3) the gene encoding glutamine synthetase. The electrophysiologic properties of cultured enteric glia were studied under whole-cell patch clamp conditions. Most cells expressed 'delayed rectifier'-type potassium currents, and some also demonstrated tetrodotoxin-sensitive sodium currents. Other subsets of voltage-dependent potassium currents, calcium currents, and glutamate-gated currents were not demonstrable.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalJournal of Neuroscience Research
Volume34
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

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Neuroglia
Potassium
Serial Passage
Glutamate-Ammonia Ligase
S100 Proteins
Cholera Toxin
Tetrodotoxin
Fibroblast Growth Factor 2
Adenylyl Cyclases
Ganglia
Glutamic Acid
Cultured Cells
Guinea Pigs
Sodium
Monoclonal Antibodies
Calcium
Phenotype
Genes

Keywords

  • cyclic AMP
  • fibroblast growth factor
  • glutamine synthetase
  • potassium channels
  • sodium channels

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Electrophysiologic and molecular properties of cultured enteric glia. / Broussard, D. L.; Bannerman, P. G C; Tang, C. M.; Hardy, M.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 34, No. 1, 1993, p. 24-31.

Research output: Contribution to journalArticle

Broussard, D. L. ; Bannerman, P. G C ; Tang, C. M. ; Hardy, M. ; Pleasure, David E. / Electrophysiologic and molecular properties of cultured enteric glia. In: Journal of Neuroscience Research. 1993 ; Vol. 34, No. 1. pp. 24-31.
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