Electrophoresis separation in open microchannels. A method for coupling electrophoresis with MALDI-MS

J. Liu, K. Tseng, B. Garcia, Carlito B Lebrilla, E. Mukerjee, S. Collins, R. Smith

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

The separation of biological mixtures in open microchannels using electrophoresis with rapid and simple coupling to mass spectrometry is introduced. Rapid open-access channel electrophoresis employs microchannels that are manufactured on microchips. Separation is performed in the open channels, and the chips are transferred to a matrix-assisted laser desorption/ionization (MALDI) source after the solvent is evaporated. The matrix (2,5-dihydroxybenzoic acid) is placed in the solution with the run buffer before the separation of the analyte components. After separation, the solvent is evaporated and the microchip is ready for MALDI-MS analysis. The microchip is placed directly into a specially designed ion source of an external source Fourier transform mass spectrometry instrument. Separation of simple mixtures containing oligosaccharides and peptides is shown.

Original languageEnglish (US)
Pages (from-to)2147-2151
Number of pages5
JournalAnalytical Chemistry
Volume73
Issue number9
DOIs
StatePublished - May 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry

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