Electropermeabilization of mammalian cells visualized with fluorescent semiconductor nanocrystals (quantum dots)

Yinghua Sun, P. Thomas Vernier, Jingjing Wang, Andras Kuthi, Laura Marcu, Martin A. Gundersen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Electroporation/electropermeabilization is a non-viral technique for gene transfection and drug delivery. Here, the transfer mechanisms were studied with fluorescent nanocrystals (quantum dots, QDs) in mammalian cells. Interactions of the cell membrane and nanoscale particles were visualized after electric pulse treatment. Responses of human multiple myeloma cells to nanocrystals were tracked for periods up to 7 days. Large particles do not cross the membrane directly after pulsing, even if the membrane is permeabilized to small molecules. Large QDs were trapped on the cell membrane for hours after electroporation and were gradually either excluded or internalized by cells. QD uptake efficiency depended on both particle size and membrane transport activity. These results, consistent with an electropermeabilization model, suggest that enhancing the interactions between the cell membrane and macromolecules may improve the transfer efficiency.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
Pages115-120
Number of pages6
Volume873
StatePublished - 2005
Event2005 MRS Spring Meeting - San Francisco, CA, United States
Duration: Mar 28 2005Apr 1 2005

Other

Other2005 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period3/28/054/1/05

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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    Sun, Y., Vernier, P. T., Wang, J., Kuthi, A., Marcu, L., & Gundersen, M. A. (2005). Electropermeabilization of mammalian cells visualized with fluorescent semiconductor nanocrystals (quantum dots). In Materials Research Society Symposium Proceedings (Vol. 873, pp. 115-120)