Electron microscopic examination of capsular material from various serotypes of Actinobacillus pleuropneumoniae.

M. Jacques, B. Foiry, Robert Higgins, K. R. Mittal

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The capsular material on PPLO broth-grown cells of Actinobacillus pleuropneumoniae representing serotypes 1 to 10 was visualized by transmission electron microscopy after polycationic ferritin labeling and also after stabilization with specific antibodies. All the isolates examined were covered with a layer of capsular material whose thickness varied between 80 to 90 nm and 210 to 230 nm when examined by immunostabilization. We were also able to visualize A. pleuropneumoniae in lungs of infected pigs and to estimate the amount of capsular material covering the cells. Our results indicate that differences in capsular structure exist among the different A. pleuropneumoniae serotypes, and this result may explain in part why the serotypes are not equally virulent.

Original languageEnglish (US)
Pages (from-to)3314-3318
Number of pages5
JournalJournal of Bacteriology
Volume170
Issue number7
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Actinobacillus pleuropneumoniae
Electrons
Mycoplasma
Transmission Electron Microscopy
Swine
Lung
Antibodies
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Electron microscopic examination of capsular material from various serotypes of Actinobacillus pleuropneumoniae. / Jacques, M.; Foiry, B.; Higgins, Robert; Mittal, K. R.

In: Journal of Bacteriology, Vol. 170, No. 7, 01.01.1988, p. 3314-3318.

Research output: Contribution to journalArticle

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