Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

Sébastien Laulhé, Tyler E. Geers, Xue Shi, Xiang Zhang, Michael H. Nantz

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31-37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.

Original languageEnglish (US)
Pages (from-to)4701-4706
Number of pages6
JournalAnalytical Methods
Volume5
Issue number18
DOIs
StatePublished - Sep 21 2013
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

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