Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17)

Lisandra L. Martin, Clemens Kubeil, Alexandr N. Simonov, Vladimir L. Kuznetsov, C. Jo Corbin, Richard J. Auchus, Alan J Conley, Alan M. Bond, Raymond J. Rodgers

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Within the superfamily of cytochrome P450 enzymes (P450s), there is a small class which is functionally employed for steroid biosynthesis. The enzymes in this class appear to have a small active site to accommodate the steroid substrates specifically and snuggly, prior to the redox transformation or hydroxylation to form a product. Cytochrome P450c17 is one of these and is also a multi-functional P450, with two activities, the first 17α-hydroxylation of pregnenolone is followed by a subsequent 17,20-lyase transformation to dehydroepiandrosterone (DHEA) as the dominant pathways to cortisol precursors or androgens in humans, respectively. How P450c17 regulates these two redox reactions is of special interest. There is a paucity of direct electrochemical studies on steroidogenic P450s, and in this mini-review we provide an overview of these studies with P450c17. Historical consideration as to the difficulties in obtaining reliable electrochemistry due to issues of handling proteins on an electrode, together with advances in the electrochemical techniques are addressed. Recent work using Fourier transformed alternating current voltammetry is highlighted as this technique can provide both catalytic information simultaneously with the underlying redox transfer with the P450 haem.

Original languageEnglish (US)
Pages (from-to)62-67
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume441
DOIs
StatePublished - Feb 5 2017

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Steroid 17-alpha-Hydroxylase
Electrochemistry
Hydroxylation
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Oxidation-Reduction
Steroids
Cytochromes
Pregnenolone
Dehydroepiandrosterone
Redox reactions
Biosynthesis
Voltammetry
Heme
Electrochemical Techniques
Androgens
Hydrocortisone
Electrodes
Catalytic Domain
Substrates

Keywords

  • Adrenal cortex
  • Androgen
  • CYP17A1
  • Cytochrome P450c17
  • Direct electrochemistry
  • Electron transfer
  • Mechanism
  • Steroidogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Martin, L. L., Kubeil, C., Simonov, A. N., Kuznetsov, V. L., Corbin, C. J., Auchus, R. J., ... Rodgers, R. J. (2017). Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17). Molecular and Cellular Endocrinology, 441, 62-67. https://doi.org/10.1016/j.mce.2016.09.034

Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17). / Martin, Lisandra L.; Kubeil, Clemens; Simonov, Alexandr N.; Kuznetsov, Vladimir L.; Corbin, C. Jo; Auchus, Richard J.; Conley, Alan J; Bond, Alan M.; Rodgers, Raymond J.

In: Molecular and Cellular Endocrinology, Vol. 441, 05.02.2017, p. 62-67.

Research output: Contribution to journalReview article

Martin, LL, Kubeil, C, Simonov, AN, Kuznetsov, VL, Corbin, CJ, Auchus, RJ, Conley, AJ, Bond, AM & Rodgers, RJ 2017, 'Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17)', Molecular and Cellular Endocrinology, vol. 441, pp. 62-67. https://doi.org/10.1016/j.mce.2016.09.034
Martin LL, Kubeil C, Simonov AN, Kuznetsov VL, Corbin CJ, Auchus RJ et al. Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17). Molecular and Cellular Endocrinology. 2017 Feb 5;441:62-67. https://doi.org/10.1016/j.mce.2016.09.034
Martin, Lisandra L. ; Kubeil, Clemens ; Simonov, Alexandr N. ; Kuznetsov, Vladimir L. ; Corbin, C. Jo ; Auchus, Richard J. ; Conley, Alan J ; Bond, Alan M. ; Rodgers, Raymond J. / Electrochemistry of cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17). In: Molecular and Cellular Endocrinology. 2017 ; Vol. 441. pp. 62-67.
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