Electrochemical desorption of proteins from gold electrode surface

Shankar Balasubramanian, Alexander Revzin, Aleksandr Simonian

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The goal of this study was to enable rational modulation of biological interfaces using electrochemical desorption of surface-bound proteins. Coupled, surface plasmon resonance (SPR) - electrochemistry instrument was used to monitor molecular assembly events on gold electrodes and to correlate these events with changes in electrochemical properties of the substrate. Model proteins, bovine serum albumin (BSA) and immunoglobulin G (IgG), were conjugated via carbodiimide (EDC) chemistry to a layer of mercaptoundecanoic acid (MUA) assembled on SPR sensor surface. Deposition of alkanethiols and proteins were monitored by ellipsometry and SPR techniques, and was further confirmed by cyclic voltammetry with potassium ferricyanide serving as a reporter molecule. The surface-bound proteins were completely removed by applying a reductive potential of -1200mV vs. Pt electrode in a physiological saline buffer. Importantly, the sequence of protein immobilization followed by desorption could be repeated multiple times, thus demonstrating ability to modulate interfacial properties. Controlled removal of protein molecules from electrode surfaces is envisioned to have important applications in affinity or cell-based biosensing, cellular micropatterning and cell sorting.

Original languageEnglish (US)
Pages (from-to)1885-1892
Number of pages8
JournalElectroanalysis
Volume18
Issue number19-20
DOIs
StatePublished - Oct 2006

Fingerprint

Gold
Desorption
Proteins
Electrodes
Surface plasmon resonance
Molecules
Ellipsometry
Electrochemistry
Bovine Serum Albumin
Electrochemical properties
Sorting
Cyclic voltammetry
Potassium
Buffers
Immunoglobulin G
Cells
Modulation
Acids
Sensors
Substrates

Keywords

  • Alkanethiols
  • Controlled removal
  • Electrochemical desorption
  • Electrochemistry
  • Surface plasmon resonance
  • Surface-bound proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Balasubramanian, S., Revzin, A., & Simonian, A. (2006). Electrochemical desorption of proteins from gold electrode surface. Electroanalysis, 18(19-20), 1885-1892. https://doi.org/10.1002/elan.200603627

Electrochemical desorption of proteins from gold electrode surface. / Balasubramanian, Shankar; Revzin, Alexander; Simonian, Aleksandr.

In: Electroanalysis, Vol. 18, No. 19-20, 10.2006, p. 1885-1892.

Research output: Contribution to journalArticle

Balasubramanian, S, Revzin, A & Simonian, A 2006, 'Electrochemical desorption of proteins from gold electrode surface', Electroanalysis, vol. 18, no. 19-20, pp. 1885-1892. https://doi.org/10.1002/elan.200603627
Balasubramanian, Shankar ; Revzin, Alexander ; Simonian, Aleksandr. / Electrochemical desorption of proteins from gold electrode surface. In: Electroanalysis. 2006 ; Vol. 18, No. 19-20. pp. 1885-1892.
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