Electrically Guiding Migration of Human Induced Pluripotent Stem Cells

Jiaping Zhang, Marco Calafiore, Qunli Zeng, Xiuzhen Zhang, Yuesheng Huang, Ronald A. Li, Wenbin Deng, Min Zhao

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A major road-block in stem cell therapy is the poor homing and integration of transplanted stem cells with the targeted host tissue. Human induced pluripotent stem (hiPS) cells are considered an excellent alternative to embryonic stem (ES) cells and we tested the feasibility of using small, physiological electric fields (EFs) to guide hiPS cells to their target. Applied EFs stimulated and guided migration of cultured hiPS cells toward the anode, with a stimulation threshold of <30 mV/mm; in three-dimensional (3D) culture hiPS cells remained stationary, whereas in an applied EF they migrated directionally. This is of significance as the therapeutic use of hiPS cells occurs in a 3D environment. EF exposure did not alter expression of the pluripotency markers SSEA-4 and Oct-4 in hiPS cells. We compared EF-directed migration (galvanotaxis) of hiPS cells and hES cells and found that hiPS cells showed greater sensitivity and directedness than those of hES cells in an EF, while hES cells migrated toward cathode. Rho-kinase (ROCK) inhibition, a method to aid expansion and survival of stem cells, significantly increased the motility, but reduced directionality of iPS cells in an EF by 70-80%. Thus, our study has revealed that physiological EF is an effective guidance cue for the migration of hiPS cells in either 2D or 3D environments and that will occur in a ROCK-dependent manner. Our current finding may lead to techniques for applying EFs in vivo to guide migration of transplanted stem cells.

Original languageEnglish (US)
Pages (from-to)987-996
Number of pages10
JournalStem Cell Reviews and Reports
Volume7
Issue number4
DOIs
StatePublished - Nov 2011

Fingerprint

Induced Pluripotent Stem Cells
Hip
Stem Cells
Electrodes
rho-Associated Kinases
Therapeutic Uses
Embryonic Stem Cells
Cell- and Tissue-Based Therapy
Cell Movement
Cues
Cultured Cells
Cell Culture Techniques

Keywords

  • Cell migration
  • Electric field (EF)
  • Galvanotaxis
  • Human induced pluripotent stem (hiPS) cell
  • Wound healing

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology

Cite this

Electrically Guiding Migration of Human Induced Pluripotent Stem Cells. / Zhang, Jiaping; Calafiore, Marco; Zeng, Qunli; Zhang, Xiuzhen; Huang, Yuesheng; Li, Ronald A.; Deng, Wenbin; Zhao, Min.

In: Stem Cell Reviews and Reports, Vol. 7, No. 4, 11.2011, p. 987-996.

Research output: Contribution to journalArticle

Zhang, Jiaping ; Calafiore, Marco ; Zeng, Qunli ; Zhang, Xiuzhen ; Huang, Yuesheng ; Li, Ronald A. ; Deng, Wenbin ; Zhao, Min. / Electrically Guiding Migration of Human Induced Pluripotent Stem Cells. In: Stem Cell Reviews and Reports. 2011 ; Vol. 7, No. 4. pp. 987-996.
@article{a23c557a17ef4212bc3564c5df6ba6b1,
title = "Electrically Guiding Migration of Human Induced Pluripotent Stem Cells",
abstract = "A major road-block in stem cell therapy is the poor homing and integration of transplanted stem cells with the targeted host tissue. Human induced pluripotent stem (hiPS) cells are considered an excellent alternative to embryonic stem (ES) cells and we tested the feasibility of using small, physiological electric fields (EFs) to guide hiPS cells to their target. Applied EFs stimulated and guided migration of cultured hiPS cells toward the anode, with a stimulation threshold of <30 mV/mm; in three-dimensional (3D) culture hiPS cells remained stationary, whereas in an applied EF they migrated directionally. This is of significance as the therapeutic use of hiPS cells occurs in a 3D environment. EF exposure did not alter expression of the pluripotency markers SSEA-4 and Oct-4 in hiPS cells. We compared EF-directed migration (galvanotaxis) of hiPS cells and hES cells and found that hiPS cells showed greater sensitivity and directedness than those of hES cells in an EF, while hES cells migrated toward cathode. Rho-kinase (ROCK) inhibition, a method to aid expansion and survival of stem cells, significantly increased the motility, but reduced directionality of iPS cells in an EF by 70-80{\%}. Thus, our study has revealed that physiological EF is an effective guidance cue for the migration of hiPS cells in either 2D or 3D environments and that will occur in a ROCK-dependent manner. Our current finding may lead to techniques for applying EFs in vivo to guide migration of transplanted stem cells.",
keywords = "Cell migration, Electric field (EF), Galvanotaxis, Human induced pluripotent stem (hiPS) cell, Wound healing",
author = "Jiaping Zhang and Marco Calafiore and Qunli Zeng and Xiuzhen Zhang and Yuesheng Huang and Li, {Ronald A.} and Wenbin Deng and Min Zhao",
year = "2011",
month = "11",
doi = "10.1007/s12015-011-9247-5",
language = "English (US)",
volume = "7",
pages = "987--996",
journal = "Stem Cell Reviews",
issn = "1550-8943",
publisher = "Humana Press",
number = "4",

}

TY - JOUR

T1 - Electrically Guiding Migration of Human Induced Pluripotent Stem Cells

AU - Zhang, Jiaping

AU - Calafiore, Marco

AU - Zeng, Qunli

AU - Zhang, Xiuzhen

AU - Huang, Yuesheng

AU - Li, Ronald A.

AU - Deng, Wenbin

AU - Zhao, Min

PY - 2011/11

Y1 - 2011/11

N2 - A major road-block in stem cell therapy is the poor homing and integration of transplanted stem cells with the targeted host tissue. Human induced pluripotent stem (hiPS) cells are considered an excellent alternative to embryonic stem (ES) cells and we tested the feasibility of using small, physiological electric fields (EFs) to guide hiPS cells to their target. Applied EFs stimulated and guided migration of cultured hiPS cells toward the anode, with a stimulation threshold of <30 mV/mm; in three-dimensional (3D) culture hiPS cells remained stationary, whereas in an applied EF they migrated directionally. This is of significance as the therapeutic use of hiPS cells occurs in a 3D environment. EF exposure did not alter expression of the pluripotency markers SSEA-4 and Oct-4 in hiPS cells. We compared EF-directed migration (galvanotaxis) of hiPS cells and hES cells and found that hiPS cells showed greater sensitivity and directedness than those of hES cells in an EF, while hES cells migrated toward cathode. Rho-kinase (ROCK) inhibition, a method to aid expansion and survival of stem cells, significantly increased the motility, but reduced directionality of iPS cells in an EF by 70-80%. Thus, our study has revealed that physiological EF is an effective guidance cue for the migration of hiPS cells in either 2D or 3D environments and that will occur in a ROCK-dependent manner. Our current finding may lead to techniques for applying EFs in vivo to guide migration of transplanted stem cells.

AB - A major road-block in stem cell therapy is the poor homing and integration of transplanted stem cells with the targeted host tissue. Human induced pluripotent stem (hiPS) cells are considered an excellent alternative to embryonic stem (ES) cells and we tested the feasibility of using small, physiological electric fields (EFs) to guide hiPS cells to their target. Applied EFs stimulated and guided migration of cultured hiPS cells toward the anode, with a stimulation threshold of <30 mV/mm; in three-dimensional (3D) culture hiPS cells remained stationary, whereas in an applied EF they migrated directionally. This is of significance as the therapeutic use of hiPS cells occurs in a 3D environment. EF exposure did not alter expression of the pluripotency markers SSEA-4 and Oct-4 in hiPS cells. We compared EF-directed migration (galvanotaxis) of hiPS cells and hES cells and found that hiPS cells showed greater sensitivity and directedness than those of hES cells in an EF, while hES cells migrated toward cathode. Rho-kinase (ROCK) inhibition, a method to aid expansion and survival of stem cells, significantly increased the motility, but reduced directionality of iPS cells in an EF by 70-80%. Thus, our study has revealed that physiological EF is an effective guidance cue for the migration of hiPS cells in either 2D or 3D environments and that will occur in a ROCK-dependent manner. Our current finding may lead to techniques for applying EFs in vivo to guide migration of transplanted stem cells.

KW - Cell migration

KW - Electric field (EF)

KW - Galvanotaxis

KW - Human induced pluripotent stem (hiPS) cell

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=82355171758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82355171758&partnerID=8YFLogxK

U2 - 10.1007/s12015-011-9247-5

DO - 10.1007/s12015-011-9247-5

M3 - Article

C2 - 21373881

AN - SCOPUS:82355171758

VL - 7

SP - 987

EP - 996

JO - Stem Cell Reviews

JF - Stem Cell Reviews

SN - 1550-8943

IS - 4

ER -