Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix

Melinda G. Simon; Sandra Lam; David D. Tran; Luis F. Alonzo; Nicholas Flohn; Steven George; Abraham P. Lee

Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix

Melinda G. Simon, Sandra Lam, David D. Tran, Luis F. Alonzo, Nicholas Flohn, Steven George, Abraham P. Lee

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Microfabricated systems are increasingly being used to recapitulate physiological environments for tissue engineering, however the accuracy of the approach requires simultaneous delivery of multiple types of stimuli to the tissue. Here, we use a microfabricated system that allows for precise fluid delivery of nutrients, coupled with an integrated electrical stimulation system, to deliver combined stimuli to iPS cell-derived cardiomyocytes in a 3-D matrix. The electrical stimulation system also offers a means by which to assess the maturity of the developing cardiomyocytes, since it can also be used to measure the maximum capture rate of beating cardiomyocytes throughout the culture period.

Original language	English (US)
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	751-753
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - Jan 1 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country	United States
City	San Antonio
Period	10/26/14 → 10/30/14

Keywords

3-D tissue
Cardiomyocytes
Induced pluripotent stem cells
Tissue engineering
Vasculogenesis

ASJC Scopus subject areas

Control and Systems Engineering

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Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix. / Simon, Melinda G.; Lam, Sandra; Tran, David D.; Alonzo, Luis F.; Flohn, Nicholas; George, Steven; Lee, Abraham P.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 751-753.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Simon, MG, Lam, S, Tran, DD, Alonzo, LF, Flohn, N, George, S & Lee, AP 2014, Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 751-753, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

Simon, Melinda G. ; Lam, Sandra ; Tran, David D. ; Alonzo, Luis F. ; Flohn, Nicholas ; George, Steven ; Lee, Abraham P. / Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 751-753

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