Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix

Melinda G. Simon, Sandra Lam, David D. Tran, Luis F. Alonzo, Nicholas Flohn, Steven George, Abraham P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microfabricated systems are increasingly being used to recapitulate physiological environments for tissue engineering, however the accuracy of the approach requires simultaneous delivery of multiple types of stimuli to the tissue. Here, we use a microfabricated system that allows for precise fluid delivery of nutrients, coupled with an integrated electrical stimulation system, to deliver combined stimuli to iPS cell-derived cardiomyocytes in a 3-D matrix. The electrical stimulation system also offers a means by which to assess the maturity of the developing cardiomyocytes, since it can also be used to measure the maximum capture rate of beating cardiomyocytes throughout the culture period.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages751-753
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • 3-D tissue
  • Cardiomyocytes
  • Induced pluripotent stem cells
  • Tissue engineering
  • Vasculogenesis

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix'. Together they form a unique fingerprint.

  • Cite this

    Simon, M. G., Lam, S., Tran, D. D., Alonzo, L. F., Flohn, N., George, S., & Lee, A. P. (2014). Electrical stimulation toward maturation of iPS cellderived cardiomyocytes in a 3-D tissue matrix. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 751-753). Chemical and Biological Microsystems Society.