Electrical properties of dog tracheal epithelial cells grown in monolayer culture.

D. L. Coleman, I. K. Tuet, J. H. Widdicombe

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Abstract

Epithelial cells from dog trachea, when grown in tissue culture, formed confluent monolayers 5-6 days after plating. After 8-10 days, domes [mean diam 356 +/- (SE) 53 micron] appeared in monolayers grown in collagen-coated flasks. When grown on polycarbonate filters coated with collagen, a detectable resistance (greater than 5 omega X cm2) and transepithelial potential difference (PD) (greater than 0.1 mV) developed 6 days after plating and increased to approximately 15 omega X cm2 and 15 mV at 10 days. Serosal ouabain (10(-4) M) abolished PD and short-circuit current (Isc). Luminal ouabain had no effect. Luminal amiloride (10(-4) M) and serosal bumetanide (10(-4) M) each decreased PD and Isc. However, a combination of both of these drugs did not abolish Isc. Isoproterenol (10(-5) M), dibutyryl adenosine 3',5'-cyclic monophosphate (10(-3) M), vasoactive intestinal peptide (10(-7) M), prostaglandin (PG) E2 (10(-5) M), PGF2 alpha (10(-5) M), and bradykinin (10(-5) M) each increased PD and Isc. Thus these monolayer cultures maintain electrical properties resembling those of the original tissue. This preparation may prove useful for the study of water and ion transport by airway epithelia.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume246
Issue number3 Pt 1
StatePublished - Mar 1984

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polycarbonate
Ouabain
Collagen
Epithelial Cells
Dogs
Bumetanide
Bucladesine
Dinoprost
Amiloride
Vasoactive Intestinal Peptide
Ion Transport
Bradykinin
Drug Combinations
Trachea
Isoproterenol
Dinoprostone
Epithelium
Water

ASJC Scopus subject areas

  • Medicine(all)

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Electrical properties of dog tracheal epithelial cells grown in monolayer culture. / Coleman, D. L.; Tuet, I. K.; Widdicombe, J. H.

In: The American journal of physiology, Vol. 246, No. 3 Pt 1, 03.1984.

Research output: Contribution to journalArticle

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