Electrical method to quantify nanoparticle interaction with lipid bilayers

Randy Carney, Yann Astier, Tamara M. Carney, Kislon Voïtchovsky, Paulo H. Jacob Silva, Francesco Stellacci

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Understanding as well as rapidly screening the interaction of nanoparticles with cell membranes is of central importance for biological applications such as drug and gene delivery. Recently, we have shown that "striped" mixed-monolayer-coated gold nanoparticles spontaneously penetrate a variety of cell membranes through a passive pathway. Here, we report an electrical approach to screen and readily quantify the interaction between nanoparticles and bilayer lipid membranes. Membrane adsorption is monitored through the capacitive increase of suspended planar lipid membranes upon fusion with nanoparticles. We adopt a Langmuir isotherm model to characterize the adsorption of nanoparticles by bilayer lipid membranes and extract the partition coefficient, K, and the standard free energy gain by this spontaneous process, for a variety of sizes of cell-membrane-penetrating nanoparticles. We believe that the method presented here will be a useful qualitative and quantitative tool to determine nanoparticle interaction with lipid bilayers and consequently with cell membranes.

Original languageEnglish (US)
Pages (from-to)932-942
Number of pages11
JournalACS Nano
Volume7
Issue number2
DOIs
StatePublished - Feb 26 2013
Externally publishedYes

Fingerprint

Lipid bilayers
lipids
Nanoparticles
nanoparticles
Cell membranes
Membrane Lipids
membranes
interactions
Adsorption
adsorption
Gold
genes
Free energy
Isotherms
partitions
Monolayers
delivery
Screening
isotherms
drugs

Keywords

  • amphiphilic nanoparticles
  • black lipid membranes
  • cell membrane penetration
  • colloidal synthesis
  • electrophysiology
  • endocytosis
  • internalization
  • planar lipid bilayers
  • surface structure

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Carney, R., Astier, Y., Carney, T. M., Voïtchovsky, K., Jacob Silva, P. H., & Stellacci, F. (2013). Electrical method to quantify nanoparticle interaction with lipid bilayers. ACS Nano, 7(2), 932-942. https://doi.org/10.1021/nn3036304

Electrical method to quantify nanoparticle interaction with lipid bilayers. / Carney, Randy; Astier, Yann; Carney, Tamara M.; Voïtchovsky, Kislon; Jacob Silva, Paulo H.; Stellacci, Francesco.

In: ACS Nano, Vol. 7, No. 2, 26.02.2013, p. 932-942.

Research output: Contribution to journalArticle

Carney, R, Astier, Y, Carney, TM, Voïtchovsky, K, Jacob Silva, PH & Stellacci, F 2013, 'Electrical method to quantify nanoparticle interaction with lipid bilayers', ACS Nano, vol. 7, no. 2, pp. 932-942. https://doi.org/10.1021/nn3036304
Carney R, Astier Y, Carney TM, Voïtchovsky K, Jacob Silva PH, Stellacci F. Electrical method to quantify nanoparticle interaction with lipid bilayers. ACS Nano. 2013 Feb 26;7(2):932-942. https://doi.org/10.1021/nn3036304
Carney, Randy ; Astier, Yann ; Carney, Tamara M. ; Voïtchovsky, Kislon ; Jacob Silva, Paulo H. ; Stellacci, Francesco. / Electrical method to quantify nanoparticle interaction with lipid bilayers. In: ACS Nano. 2013 ; Vol. 7, No. 2. pp. 932-942.
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