Electrical Guidance of Human Stem Cells in the Rat Brain

Jun Feng Feng, Jing Liu, Lei Zhang, Ji Yao Jiang, Michael Russell, Bruce G Lyeth, Jan Nolta, Min Zhao

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Limited migration of neural stem cells in adult brain is a roadblock for the use of stem cell therapies to treat brain diseases and injuries. Here, we report a strategy that mobilizes and guides migration of stem cells in the brain in vivo. We developed a safe stimulation paradigm to deliver directional currents in the brain. Tracking cells expressing GFP demonstrated electrical mobilization and guidance of migration of human neural stem cells, even against co-existing intrinsic cues in the rostral migration stream. Transplanted cells were observed at 3 weeks and 4 months after stimulation in areas guided by the stimulation currents, and with indications of differentiation. Electrical stimulation thus may provide a potential approach to facilitate brain stem cell therapies.

Original languageEnglish (US)
Pages (from-to)177-189
Number of pages13
JournalStem Cell Reports
Volume9
Issue number1
DOIs
StatePublished - Jul 11 2017

Fingerprint

Stem cells
Rats
Brain
Stem Cells
Neural Stem Cells
Cell- and Tissue-Based Therapy
Cell Tracking
Brain Diseases
Brain Injuries
Electric Stimulation
Brain Stem
Cues
Cells

Keywords

  • brain in vivo
  • directional cell migration
  • electric field
  • electric stimulation
  • electrotaxis
  • galvanotaxis
  • human neural stem cells
  • in vivo migration
  • neural stem cells
  • neuroblasts

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Electrical Guidance of Human Stem Cells in the Rat Brain. / Feng, Jun Feng; Liu, Jing; Zhang, Lei; Jiang, Ji Yao; Russell, Michael; Lyeth, Bruce G; Nolta, Jan; Zhao, Min.

In: Stem Cell Reports, Vol. 9, No. 1, 11.07.2017, p. 177-189.

Research output: Contribution to journalArticle

Feng, Jun Feng ; Liu, Jing ; Zhang, Lei ; Jiang, Ji Yao ; Russell, Michael ; Lyeth, Bruce G ; Nolta, Jan ; Zhao, Min. / Electrical Guidance of Human Stem Cells in the Rat Brain. In: Stem Cell Reports. 2017 ; Vol. 9, No. 1. pp. 177-189.
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