Elastin synthesis during perinatal lung development in the rat

Barbara Myers, Michael Dubick, Jerold A Last, Robert B. Rucker

Research output: Contribution to journalArticle

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Abstract

The rate of soluble elastin synthesis was estimated in lung explants from rats of differing ages to better define periods in lung development important to the deposition of lung elastin. Lungs from rat pups at days 1, 3, 7, 9, 12, 15, and 21 post-parturition and from adult rats were incubated in a defined medium containing L-[3H]valine. Following incubation, labelled soluble elastin (tropoelastin) was separated from other soluble proteins by coacervation and electrophoresis on polyacrylamide gels containing sodium dodecyl sulfate. The tropoelastin synthetic rate was then estimated after correcting for differences in recovery of radioactivity as tropoelastin and lung tissue L-[3H]valine specific activity. Maximal rates of elastin synthesis were observed in lung explants from 7-12-day-old rats. The rate of elastin synthesis during this period was 5-8-times the rate observed in adult rat lung (expressed per g of fresh lung) and represented approx. 2% of the total protein ynthesis. Moreover, the values derived from explant culture for elastin synthesis were consistent with values for lung elastin deposition in the perinatal rat (5-10 μg elastin/h per g lung).

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalBBA - General Subjects
Volume761
Issue number1
DOIs
StatePublished - Nov 22 1983

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Keywords

  • (Rat lung)
  • Elastin synthesis
  • Lung development
  • Tropoelastin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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