Ehrlichia SPP. in cervids from California

Janet E Foley, Jeffrey E. Barlough, Robert B. Kimsey, John E Madigan, Elfriede DeRock, Amy Poland

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Blood samples from six mule deer (Odocoileus hemionus hemionus), 15 black-tailed deer (O. hemionus columbianus), and 29 elk (Cervus elaphus nannodes) were assayed for human monocytic and human granulocytic ehrlichiosis (HGE) by polymerase chain reaction (PCR), DNA sequencing, and serology to determine whether or not cervids are involved in the maintenance of these potential human pathogens in California (USA). The deer were sampled in August to October 1992-95. The 29 tule elk from Point Reyes National Seashore were sampled in August 1997. All deer were seronegative for antibodies to HGE/Ehrlichia equi, while the E. equi sero-prevalence among elk was 17%. The 16S rDNA PCR prevalence in deer was 38% (in mule deer and black-tailed deer) for Ehrlichia-like sp. of white-tailed deer, 5% (one black-tailed deer only) for E. equi, and 0% for E. chaffeensis. The PCR prevalence in elk was 0% for Ehrlichia-like sp. of white-tailed deer, 31% for E. equi, and 0% for E. chaffeensis. The E. equi from two positive elk samples was successfully propagated in HL-60 cell cultures. DNA sequencing confirmed that the Ehrlichia-like sp. sequences from deer in California were closely related to sequences reported from white-tailed deer from Oklahoma and Georgia. The E. equi strain from deer and elk resembled other E. equi strains from California. These results suggest that cervids may be important in the natural maintenance of E. equi in California.

Original languageEnglish (US)
Pages (from-to)731-737
Number of pages7
JournalJournal of Wildlife Diseases
Volume34
Issue number4
StatePublished - Oct 1998

Fingerprint

Ehrlichia
Anaplasma phagocytophilum
Deer
Odocoileus hemionus
deer
elks
human granulocytic ehrlichiosis
Odocoileus virginianus
polymerase chain reaction
Ehrlichiosis
sequence analysis
Equidae
DNA Sequence Analysis
Polymerase Chain Reaction
Cervus elaphus
seroprevalence
cell culture
DNA
HL-60 Cells
Serology

Keywords

  • Cervus elaphus nannodes
  • Ehrlichia equi
  • Human granulocytic ehrlichiosis
  • Odocoileus hemionus columbianus
  • Odocoileus hemionus hemionus
  • Survey

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Foley, J. E., Barlough, J. E., Kimsey, R. B., Madigan, J. E., DeRock, E., & Poland, A. (1998). Ehrlichia SPP. in cervids from California. Journal of Wildlife Diseases, 34(4), 731-737.

Ehrlichia SPP. in cervids from California. / Foley, Janet E; Barlough, Jeffrey E.; Kimsey, Robert B.; Madigan, John E; DeRock, Elfriede; Poland, Amy.

In: Journal of Wildlife Diseases, Vol. 34, No. 4, 10.1998, p. 731-737.

Research output: Contribution to journalArticle

Foley, JE, Barlough, JE, Kimsey, RB, Madigan, JE, DeRock, E & Poland, A 1998, 'Ehrlichia SPP. in cervids from California', Journal of Wildlife Diseases, vol. 34, no. 4, pp. 731-737.
Foley JE, Barlough JE, Kimsey RB, Madigan JE, DeRock E, Poland A. Ehrlichia SPP. in cervids from California. Journal of Wildlife Diseases. 1998 Oct;34(4):731-737.
Foley, Janet E ; Barlough, Jeffrey E. ; Kimsey, Robert B. ; Madigan, John E ; DeRock, Elfriede ; Poland, Amy. / Ehrlichia SPP. in cervids from California. In: Journal of Wildlife Diseases. 1998 ; Vol. 34, No. 4. pp. 731-737.
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abstract = "Blood samples from six mule deer (Odocoileus hemionus hemionus), 15 black-tailed deer (O. hemionus columbianus), and 29 elk (Cervus elaphus nannodes) were assayed for human monocytic and human granulocytic ehrlichiosis (HGE) by polymerase chain reaction (PCR), DNA sequencing, and serology to determine whether or not cervids are involved in the maintenance of these potential human pathogens in California (USA). The deer were sampled in August to October 1992-95. The 29 tule elk from Point Reyes National Seashore were sampled in August 1997. All deer were seronegative for antibodies to HGE/Ehrlichia equi, while the E. equi sero-prevalence among elk was 17{\%}. The 16S rDNA PCR prevalence in deer was 38{\%} (in mule deer and black-tailed deer) for Ehrlichia-like sp. of white-tailed deer, 5{\%} (one black-tailed deer only) for E. equi, and 0{\%} for E. chaffeensis. The PCR prevalence in elk was 0{\%} for Ehrlichia-like sp. of white-tailed deer, 31{\%} for E. equi, and 0{\%} for E. chaffeensis. The E. equi from two positive elk samples was successfully propagated in HL-60 cell cultures. DNA sequencing confirmed that the Ehrlichia-like sp. sequences from deer in California were closely related to sequences reported from white-tailed deer from Oklahoma and Georgia. The E. equi strain from deer and elk resembled other E. equi strains from California. These results suggest that cervids may be important in the natural maintenance of E. equi in California.",
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