EGF and TGF-α supplementation enhances development of cloned mouse embryos

Tedla D. Dadi, Ming Wen Li, Kevin C K Lloyd

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, we sought to determine the extent to which mitogenic growth factors affect the survival and development of cloned mouse embryos in vitro. Cloned embryos derived by intracytoplasmic nuclear injection (ICNI) of cumulus cell nuclei into enucleated oocytes were incubated in culture media supplemented with EGF and/or TGF-α for 4 days. Compared to control, treatment with either growth factor significantly increased the blastocyst formation rate, the total number of cells per blastocyst, the cell ratio of the inner cell mass and the trophectoderm (ICM:TE ratio), and EGF-R protein expression in cloned embryos. In most instances these effects were enhanced in cloned embryos when EGF and TGF-α were combined. Although fewer blastocysts developed from cloned than from fertilized one-cell stage embryos, growth factor treatment appeared to have the greatest effect on cloned embryos. These results demonstrate that mitogenic growth factors significantly enhance survival and promote the preimplantation development of cloned mouse embryos.

Original languageEnglish (US)
Pages (from-to)315-326
Number of pages12
JournalCloning and Stem Cells
Volume9
Issue number3
DOIs
StatePublished - Sep 1 2007

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Epidermal Growth Factor
Embryonic Structures
Intercellular Signaling Peptides and Proteins
Blastocyst
Cumulus Cells
Cell Nucleus
Oocytes
Culture Media
Cell Count
Injections
Therapeutics
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Applied Microbiology and Biotechnology

Cite this

EGF and TGF-α supplementation enhances development of cloned mouse embryos. / Dadi, Tedla D.; Li, Ming Wen; Lloyd, Kevin C K.

In: Cloning and Stem Cells, Vol. 9, No. 3, 01.09.2007, p. 315-326.

Research output: Contribution to journalArticle

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