Abstract
In this study, we sought to determine the extent to which mitogenic growth factors affect the survival and development of cloned mouse embryos in vitro. Cloned embryos derived by intracytoplasmic nuclear injection (ICNI) of cumulus cell nuclei into enucleated oocytes were incubated in culture media supplemented with EGF and/or TGF-α for 4 days. Compared to control, treatment with either growth factor significantly increased the blastocyst formation rate, the total number of cells per blastocyst, the cell ratio of the inner cell mass and the trophectoderm (ICM:TE ratio), and EGF-R protein expression in cloned embryos. In most instances these effects were enhanced in cloned embryos when EGF and TGF-α were combined. Although fewer blastocysts developed from cloned than from fertilized one-cell stage embryos, growth factor treatment appeared to have the greatest effect on cloned embryos. These results demonstrate that mitogenic growth factors significantly enhance survival and promote the preimplantation development of cloned mouse embryos.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 315-326 |
| Number of pages | 12 |
| Journal | Cloning and Stem Cells |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2007 |
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ASJC Scopus subject areas
- Biotechnology
- Developmental Biology
- Applied Microbiology and Biotechnology
Cite this
EGF and TGF-α supplementation enhances development of cloned mouse embryos. / Dadi, Tedla D.; Li, Ming Wen; Lloyd, Kevin C K.
In: Cloning and Stem Cells, Vol. 9, No. 3, 01.09.2007, p. 315-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - EGF and TGF-α supplementation enhances development of cloned mouse embryos
AU - Dadi, Tedla D.
AU - Li, Ming Wen
AU - Lloyd, Kevin C K
PY - 2007/9/1
Y1 - 2007/9/1
N2 - In this study, we sought to determine the extent to which mitogenic growth factors affect the survival and development of cloned mouse embryos in vitro. Cloned embryos derived by intracytoplasmic nuclear injection (ICNI) of cumulus cell nuclei into enucleated oocytes were incubated in culture media supplemented with EGF and/or TGF-α for 4 days. Compared to control, treatment with either growth factor significantly increased the blastocyst formation rate, the total number of cells per blastocyst, the cell ratio of the inner cell mass and the trophectoderm (ICM:TE ratio), and EGF-R protein expression in cloned embryos. In most instances these effects were enhanced in cloned embryos when EGF and TGF-α were combined. Although fewer blastocysts developed from cloned than from fertilized one-cell stage embryos, growth factor treatment appeared to have the greatest effect on cloned embryos. These results demonstrate that mitogenic growth factors significantly enhance survival and promote the preimplantation development of cloned mouse embryos.
AB - In this study, we sought to determine the extent to which mitogenic growth factors affect the survival and development of cloned mouse embryos in vitro. Cloned embryos derived by intracytoplasmic nuclear injection (ICNI) of cumulus cell nuclei into enucleated oocytes were incubated in culture media supplemented with EGF and/or TGF-α for 4 days. Compared to control, treatment with either growth factor significantly increased the blastocyst formation rate, the total number of cells per blastocyst, the cell ratio of the inner cell mass and the trophectoderm (ICM:TE ratio), and EGF-R protein expression in cloned embryos. In most instances these effects were enhanced in cloned embryos when EGF and TGF-α were combined. Although fewer blastocysts developed from cloned than from fertilized one-cell stage embryos, growth factor treatment appeared to have the greatest effect on cloned embryos. These results demonstrate that mitogenic growth factors significantly enhance survival and promote the preimplantation development of cloned mouse embryos.
UR - http://www.scopus.com/inward/record.url?scp=41049095640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41049095640&partnerID=8YFLogxK
U2 - 10.1089/clo.2006.0040
DO - 10.1089/clo.2006.0040
M3 - Article
C2 - 17907942
AN - SCOPUS:41049095640
VL - 9
SP - 315
EP - 326
JO - Cellular Reprogramming
JF - Cellular Reprogramming
SN - 2152-4971
IS - 3
ER -