Efficient recombinant of Lym-1 scFv gene using multiple doubly- restricted DNA fragments

Xu Bao Shi, Paul H. Gumerlock, Linda Kroger, Gerald L Denardo, Sally J. DeNardo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to improve radioimmunotherapy of lymphoma, a Lym-1 single- chain antigen-binding (scFv) protein molecule was produced. Because the commonly used polymerase chain reaction (PCR) method frequently causes unexpected mutations, we developed a non-PCR method for scFv gene assembly. The method involved a stepwise of doubly-restricted DNA fragments and re- digestion of the resultant concatamers. Using this strategy, the Lym-1 scFv expression gene was readily constructed without mutations. The recombinant gene was cloned into an expression vector and scFv protein was expressed. The method can be used for other genes or DNA recombination.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalCancer Biotherapy and Radiopharmaceuticals
Volume14
Issue number2
StatePublished - 1999

Fingerprint

DNA
Genes
Radioimmunotherapy
Mutation
Genetic Recombination
Digestion
Lymphoma
Carrier Proteins
Gene Expression
Antigens
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Oncology

Cite this

Efficient recombinant of Lym-1 scFv gene using multiple doubly- restricted DNA fragments. / Shi, Xu Bao; Gumerlock, Paul H.; Kroger, Linda; Denardo, Gerald L; DeNardo, Sally J.

In: Cancer Biotherapy and Radiopharmaceuticals, Vol. 14, No. 2, 1999, p. 139-143.

Research output: Contribution to journalArticle

Shi, Xu Bao ; Gumerlock, Paul H. ; Kroger, Linda ; Denardo, Gerald L ; DeNardo, Sally J. / Efficient recombinant of Lym-1 scFv gene using multiple doubly- restricted DNA fragments. In: Cancer Biotherapy and Radiopharmaceuticals. 1999 ; Vol. 14, No. 2. pp. 139-143.
@article{6c3a7fd8a596441d8b63fd28f68db6ef,
title = "Efficient recombinant of Lym-1 scFv gene using multiple doubly- restricted DNA fragments",
abstract = "In order to improve radioimmunotherapy of lymphoma, a Lym-1 single- chain antigen-binding (scFv) protein molecule was produced. Because the commonly used polymerase chain reaction (PCR) method frequently causes unexpected mutations, we developed a non-PCR method for scFv gene assembly. The method involved a stepwise of doubly-restricted DNA fragments and re- digestion of the resultant concatamers. Using this strategy, the Lym-1 scFv expression gene was readily constructed without mutations. The recombinant gene was cloned into an expression vector and scFv protein was expressed. The method can be used for other genes or DNA recombination.",
author = "Shi, {Xu Bao} and Gumerlock, {Paul H.} and Linda Kroger and Denardo, {Gerald L} and DeNardo, {Sally J.}",
year = "1999",
language = "English (US)",
volume = "14",
pages = "139--143",
journal = "Cancer Biotherapy and Radiopharmaceuticals",
issn = "1084-9785",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

TY - JOUR

T1 - Efficient recombinant of Lym-1 scFv gene using multiple doubly- restricted DNA fragments

AU - Shi, Xu Bao

AU - Gumerlock, Paul H.

AU - Kroger, Linda

AU - Denardo, Gerald L

AU - DeNardo, Sally J.

PY - 1999

Y1 - 1999

N2 - In order to improve radioimmunotherapy of lymphoma, a Lym-1 single- chain antigen-binding (scFv) protein molecule was produced. Because the commonly used polymerase chain reaction (PCR) method frequently causes unexpected mutations, we developed a non-PCR method for scFv gene assembly. The method involved a stepwise of doubly-restricted DNA fragments and re- digestion of the resultant concatamers. Using this strategy, the Lym-1 scFv expression gene was readily constructed without mutations. The recombinant gene was cloned into an expression vector and scFv protein was expressed. The method can be used for other genes or DNA recombination.

AB - In order to improve radioimmunotherapy of lymphoma, a Lym-1 single- chain antigen-binding (scFv) protein molecule was produced. Because the commonly used polymerase chain reaction (PCR) method frequently causes unexpected mutations, we developed a non-PCR method for scFv gene assembly. The method involved a stepwise of doubly-restricted DNA fragments and re- digestion of the resultant concatamers. Using this strategy, the Lym-1 scFv expression gene was readily constructed without mutations. The recombinant gene was cloned into an expression vector and scFv protein was expressed. The method can be used for other genes or DNA recombination.

UR - http://www.scopus.com/inward/record.url?scp=0032586390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032586390&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 139

EP - 143

JO - Cancer Biotherapy and Radiopharmaceuticals

JF - Cancer Biotherapy and Radiopharmaceuticals

SN - 1084-9785

IS - 2

ER -