Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

Kyle J. Lauersen, Thomas Baier, Julian Wichmann, Robin Wördenweber, Jan H. Mussgnug, Wolfgang Hübner, Thomas R Huser, Olaf Kruse

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922±242 µg g−1 CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03 mg L−1 sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source.

Original languageEnglish (US)
Pages (from-to)331-343
Number of pages13
JournalMetabolic Engineering
Volume38
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

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Keywords

  • Chlamydomonas reinhardtii
  • Microalgae
  • Patchoulol
  • Sesquiterpenoids
  • Terpenoids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Lauersen, K. J., Baier, T., Wichmann, J., Wördenweber, R., Mussgnug, J. H., Hübner, W., Huser, T. R., & Kruse, O. (2016). Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii. Metabolic Engineering, 38, 331-343. https://doi.org/10.1016/j.ymben.2016.07.013