Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li; Yonghui Liu; Yue Wan; Yanhong Li; Xi Chen; Wei Zhao; Peng George Wang

doi:10.1021/ol402585c

Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li, Yonghui Liu, Yue Wan, Yanhong Li, Xi Chen, Wei Zhao, Peng George Wang

Chemistry

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

Original language	English (US)
Pages (from-to)	5528-5530
Number of pages	3
Journal	Organic Letters
Volume	15
Issue number	21
DOIs	https://doi.org/10.1021/ol402585c
State	Published - Nov 1 2013

ASJC Scopus subject areas

Organic Chemistry
Physical and Theoretical Chemistry
Biochemistry

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title = "Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives",

abstract = "An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.",

author = "Lei Li and Yonghui Liu and Yue Wan and Yanhong Li and Xi Chen and Wei Zhao and Wang, {Peng George}",

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month = nov,

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doi = "10.1021/ol402585c",

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volume = "15",

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journal = "Organic Letters",

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T1 - Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

AU - Li, Lei

AU - Liu, Yonghui

AU - Wan, Yue

AU - Li, Yanhong

AU - Chen, Xi

AU - Zhao, Wei

AU - Wang, Peng George

PY - 2013/11/1

Y1 - 2013/11/1

N2 - An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

AB - An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

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