Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li; Yonghui Liu; Yue Wan; Yanhong Li; Xi Chen; Wei Zhao; Peng George Wang

doi:10.1021/ol402585c

Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li, Yonghui Liu, Yue Wan, Yanhong Li, Xi Chen, Wei Zhao, Peng George Wang

Chemistry

Research output: Contribution to journal › Article

19 Citations (Scopus)

Abstract

An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

Original language	English (US)
Pages (from-to)	5528-5530
Number of pages	3
Journal	Organic Letters
Volume	15
Issue number	21
DOIs	https://doi.org/10.1021/ol402585c
State	Published - Nov 1 2013

Fingerprint

Guanosine Diphosphate Sugars

Guanosine Diphosphate Mannose

guanosines

diphosphates

Guanosine

Diphosphates

sugars

Sugars

Pyrococcus furiosus

Inorganic Pyrophosphatase

Derivatives

Monosaccharides

synthesis

Phosphotransferases

Escherichia coli

monosaccharides

Enzymes

Mannose

Escherichia

glucose

ASJC Scopus subject areas

Organic Chemistry
Physical and Theoretical Chemistry
Biochemistry

Cite this

Li, L., Liu, Y., Wan, Y., Li, Y., Chen, X., Zhao, W., & Wang, P. G. (2013). Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. Organic Letters, 15(21), 5528-5530. https://doi.org/10.1021/ol402585c

Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. / Li, Lei; Liu, Yonghui; Wan, Yue; Li, Yanhong; Chen, Xi; Zhao, Wei; Wang, Peng George.

In: Organic Letters, Vol. 15, No. 21, 01.11.2013, p. 5528-5530.

Research output: Contribution to journal › Article

Li, L, Liu, Y, Wan, Y, Li, Y, Chen, X, Zhao, W & Wang, PG 2013, 'Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives', Organic Letters, vol. 15, no. 21, pp. 5528-5530. https://doi.org/10.1021/ol402585c

Li L, Liu Y, Wan Y, Li Y, Chen X, Zhao W et al. Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. Organic Letters. 2013 Nov 1;15(21):5528-5530. https://doi.org/10.1021/ol402585c

Li, Lei ; Liu, Yonghui ; Wan, Yue ; Li, Yanhong ; Chen, Xi ; Zhao, Wei ; Wang, Peng George. / Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. In: Organic Letters. 2013 ; Vol. 15, No. 21. pp. 5528-5530.

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10.1021/ol402585c