Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li, Yonghui Liu, Yue Wan, Yanhong Li, Xi Chen, Wei Zhao, Peng George Wang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

Original languageEnglish (US)
Pages (from-to)5528-5530
Number of pages3
JournalOrganic Letters
Volume15
Issue number21
DOIs
StatePublished - Nov 1 2013

Fingerprint

Guanosine Diphosphate Sugars
Guanosine Diphosphate Mannose
guanosines
diphosphates
Guanosine
Diphosphates
sugars
Sugars
Pyrococcus furiosus
Inorganic Pyrophosphatase
Derivatives
Monosaccharides
synthesis
Phosphotransferases
Escherichia coli
monosaccharides
Enzymes
Mannose
Escherichia
glucose

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Biochemistry

Cite this

Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. / Li, Lei; Liu, Yonghui; Wan, Yue; Li, Yanhong; Chen, Xi; Zhao, Wei; Wang, Peng George.

In: Organic Letters, Vol. 15, No. 21, 01.11.2013, p. 5528-5530.

Research output: Contribution to journalArticle

Li, L, Liu, Y, Wan, Y, Li, Y, Chen, X, Zhao, W & Wang, PG 2013, 'Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives', Organic Letters, vol. 15, no. 21, pp. 5528-5530. https://doi.org/10.1021/ol402585c
Li, Lei ; Liu, Yonghui ; Wan, Yue ; Li, Yanhong ; Chen, Xi ; Zhao, Wei ; Wang, Peng George. / Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. In: Organic Letters. 2013 ; Vol. 15, No. 21. pp. 5528-5530.
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