Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li; Yonghui Liu; Yue Wan; Yanhong Li; Xi Chen; Wei Zhao; Peng George Wang

doi:10.1021/ol402585c

Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

Lei Li, Yonghui Liu, Yue Wan, Yanhong Li, Xi Chen, Wei Zhao, Peng George Wang

Chemistry

Research output: Contribution to journal › Article

20 Scopus citations

Abstract

An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

Original language	English (US)
Pages (from-to)	5528-5530
Number of pages	3
Journal	Organic Letters
Volume	15
Issue number	21
DOIs	https://doi.org/10.1021/ol402585c
State	Published - Nov 1 2013

Fingerprint

ASJC Scopus subject areas

Organic Chemistry
Physical and Theoretical Chemistry
Biochemistry

Cite this

Li, L., Liu, Y., Wan, Y., Li, Y., Chen, X., Zhao, W., & Wang, P. G. (2013). Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives. Organic Letters, 15(21), 5528-5530. https://doi.org/10.1021/ol402585c

@article{b39909c0dba64cbb94331a7917534d31,

title = "Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives",

abstract = "An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.",

author = "Lei Li and Yonghui Liu and Yue Wan and Yanhong Li and Xi Chen and Wei Zhao and Wang, {Peng George}",

year = "2013",

month = nov

day = "1",

doi = "10.1021/ol402585c",

language = "English (US)",

volume = "15",

pages = "5528--5530",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "21",

}

TY - JOUR

T1 - Efficient enzymatic synthesis of guanosine 5′-diphosphate-sugars and derivatives

AU - Li, Lei

AU - Liu, Yonghui

AU - Wan, Yue

AU - Li, Yanhong

AU - Chen, Xi

AU - Zhao, Wei

AU - Wang, Peng George

PY - 2013/11/1

Y1 - 2013/11/1

N2 - An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

AB - An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK-15697), a guanosine 5′-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

UR - http://www.scopus.com/inward/record.url?scp=84887035731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887035731&partnerID=8YFLogxK

U2 - 10.1021/ol402585c

DO - 10.1021/ol402585c

M3 - Article

C2 - 24117142

AN - SCOPUS:84887035731

VL - 15

SP - 5528

EP - 5530

JO - Organic Letters

JF - Organic Letters

SN - 1523-7060

IS - 21

ER -

Access to Document

10.1021/ol402585c