Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

Lei Li, Yonghui Liu, Tiehai Li, Wenjun Wang, Zaikuan Yu, Cheng Ma, Jingyao Qu, Wei Zhao, Xi Chen, Peng G. Wang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.

Original languageEnglish (US)
Pages (from-to)10310-10313
Number of pages4
JournalChemical Communications
Volume51
Issue number51
DOIs
StatePublished - Jun 28 2015

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Sialyltransferases
Enzymes
Derivatives
N-Acylneuraminate Cytidylyltransferase
Galactokinase
Hexosamines
Phosphorylases
Galactose
Acids

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms. / Li, Lei; Liu, Yonghui; Li, Tiehai; Wang, Wenjun; Yu, Zaikuan; Ma, Cheng; Qu, Jingyao; Zhao, Wei; Chen, Xi; Wang, Peng G.

In: Chemical Communications, Vol. 51, No. 51, 28.06.2015, p. 10310-10313.

Research output: Contribution to journalArticle

Li, L, Liu, Y, Li, T, Wang, W, Yu, Z, Ma, C, Qu, J, Zhao, W, Chen, X & Wang, PG 2015, 'Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms', Chemical Communications, vol. 51, no. 51, pp. 10310-10313. https://doi.org/10.1039/c5cc03746h
Li, Lei ; Liu, Yonghui ; Li, Tiehai ; Wang, Wenjun ; Yu, Zaikuan ; Ma, Cheng ; Qu, Jingyao ; Zhao, Wei ; Chen, Xi ; Wang, Peng G. / Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms. In: Chemical Communications. 2015 ; Vol. 51, No. 51. pp. 10310-10313.
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AU - Ma, Cheng

AU - Qu, Jingyao

AU - Zhao, Wei

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