Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

Lei Li; Yonghui Liu; Tiehai Li; Wenjun Wang; Zaikuan Yu; Cheng Ma; Jingyao Qu; Wei Zhao; Xi Chen; Peng G. Wang

doi:10.1039/c5cc03746h

Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

Lei Li, Yonghui Liu, Tiehai Li, Wenjun Wang, Zaikuan Yu, Cheng Ma, Jingyao Qu, Wei Zhao, Xi Chen, Peng G. Wang

Chemistry

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.

Original language	English (US)
Pages (from-to)	10310-10313
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	51
DOIs	https://doi.org/10.1039/c5cc03746h
State	Published - Jun 28 2015

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms",

abstract = "Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.",

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T1 - Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

AU - Li, Lei

AU - Liu, Yonghui

AU - Li, Tiehai

AU - Wang, Wenjun

AU - Yu, Zaikuan

AU - Ma, Cheng

AU - Qu, Jingyao

AU - Zhao, Wei

AU - Chen, Xi

AU - Wang, Peng G.

PY - 2015/6/28

Y1 - 2015/6/28

N2 - Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.

AB - Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.

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Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

Abstract

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