Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids

Hai Yu, Harshal A. Chokhawala, Ajit Varki, Xi Chen

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups. This journal is

Original languageEnglish (US)
Pages (from-to)2458-2463
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume5
Issue number15
DOIs
StatePublished - 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)

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