Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids

Hai Yu, Harshal A. Chokhawala, Ajit Varki, Xi Chen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups. This journal is

Original languageEnglish (US)
Pages (from-to)2458-2463
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume5
Issue number15
DOIs
StatePublished - 2007

Fingerprint

Sialic Acids
N-Acetylneuraminic Acid
albumins
Serum Albumin
serums
Esters
Carbohydrates
Derivatives
acids
carbohydrates
Enzymes
synthesis
reagents
enzymes
esters
Proteins
proteins
adipic acid
4-nitrophenyl
lactosides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)

Cite this

Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. / Yu, Hai; Chokhawala, Harshal A.; Varki, Ajit; Chen, Xi.

In: Organic and Biomolecular Chemistry, Vol. 5, No. 15, 2007, p. 2458-2463.

Research output: Contribution to journalArticle

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