Abstract
Aspergillus fumigatus causes disease in birds. Our objectives were to determine pharmacokinetic parameters and evaluate efficacy of voriconazole (VCZ) in a novel experimental quail model. After a single oral VCZ dose of 20 or 40 mg/kg, plasma concentrations peaked 2 h postdose (5.8 and 6.9 μg/ml) and remained above 0.5 μg/ml for 4 and 12 h postdose, respectively. For the efficacy study, ten-day-old Japanese quail (n 60) were infected intratracheally with A. fumigatus conidia. Daily oral VCZ at 20 or 40 mg/kg was initiated 24 h postinfection (PI); infected diluent-treated birds were given de-ionized water orally. Preassigned birds were euthanized on days 5 or 10 PI. VCZ at 40 mg/kg prolonged survival compared to 20 mg/kg or diluent-treatment (P < 0.05) and lungs from birds given VCZ at 40 mg/kg had fewer colony forming units (CFU) than diluent-treated (P =0.03). At day 10 PI, birds treated with VCZ at 20 mg/kg had significantly fewer fungi in the lungs as demonstrated by methenamine silver stain (P =0.017) or immunohistochemistry, as compared to diluent-treated (P =0.034). Histopathologically, VCZ-treated birds did not have necrotic lesions and showed a trend toward fewer with acute inflammatory changes. VCZ at 40 mg/kg was efficacious in quail with experimental pulmonary aspergillosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 234-244 |
| Number of pages | 11 |
| Journal | Medical Mycology |
| Volume | 48 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2010 |
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Keywords
- Antifungal
- Aspergillosis
- Avian
- Fungal infection
- Voriconazole
ASJC Scopus subject areas
- veterinary(all)
- Infectious Diseases
Cite this
Efficacy of voriconazole in Japanese quail (Coturnix japonica) experimentally infected with Aspergillus fumigatus. / Tell, Lisa A; Clemons, Karl V.; Kline, Yvonne; Woods, Leslie; Kass, Philip H; Martinez, Marife; Stevens, David A.
In: Medical Mycology, Vol. 48, No. 2, 2010, p. 234-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacy of voriconazole in Japanese quail (Coturnix japonica) experimentally infected with Aspergillus fumigatus
AU - Tell, Lisa A
AU - Clemons, Karl V.
AU - Kline, Yvonne
AU - Woods, Leslie
AU - Kass, Philip H
AU - Martinez, Marife
AU - Stevens, David A.
PY - 2010
Y1 - 2010
N2 - Aspergillus fumigatus causes disease in birds. Our objectives were to determine pharmacokinetic parameters and evaluate efficacy of voriconazole (VCZ) in a novel experimental quail model. After a single oral VCZ dose of 20 or 40 mg/kg, plasma concentrations peaked 2 h postdose (5.8 and 6.9 μg/ml) and remained above 0.5 μg/ml for 4 and 12 h postdose, respectively. For the efficacy study, ten-day-old Japanese quail (n 60) were infected intratracheally with A. fumigatus conidia. Daily oral VCZ at 20 or 40 mg/kg was initiated 24 h postinfection (PI); infected diluent-treated birds were given de-ionized water orally. Preassigned birds were euthanized on days 5 or 10 PI. VCZ at 40 mg/kg prolonged survival compared to 20 mg/kg or diluent-treatment (P < 0.05) and lungs from birds given VCZ at 40 mg/kg had fewer colony forming units (CFU) than diluent-treated (P =0.03). At day 10 PI, birds treated with VCZ at 20 mg/kg had significantly fewer fungi in the lungs as demonstrated by methenamine silver stain (P =0.017) or immunohistochemistry, as compared to diluent-treated (P =0.034). Histopathologically, VCZ-treated birds did not have necrotic lesions and showed a trend toward fewer with acute inflammatory changes. VCZ at 40 mg/kg was efficacious in quail with experimental pulmonary aspergillosis.
AB - Aspergillus fumigatus causes disease in birds. Our objectives were to determine pharmacokinetic parameters and evaluate efficacy of voriconazole (VCZ) in a novel experimental quail model. After a single oral VCZ dose of 20 or 40 mg/kg, plasma concentrations peaked 2 h postdose (5.8 and 6.9 μg/ml) and remained above 0.5 μg/ml for 4 and 12 h postdose, respectively. For the efficacy study, ten-day-old Japanese quail (n 60) were infected intratracheally with A. fumigatus conidia. Daily oral VCZ at 20 or 40 mg/kg was initiated 24 h postinfection (PI); infected diluent-treated birds were given de-ionized water orally. Preassigned birds were euthanized on days 5 or 10 PI. VCZ at 40 mg/kg prolonged survival compared to 20 mg/kg or diluent-treatment (P < 0.05) and lungs from birds given VCZ at 40 mg/kg had fewer colony forming units (CFU) than diluent-treated (P =0.03). At day 10 PI, birds treated with VCZ at 20 mg/kg had significantly fewer fungi in the lungs as demonstrated by methenamine silver stain (P =0.017) or immunohistochemistry, as compared to diluent-treated (P =0.034). Histopathologically, VCZ-treated birds did not have necrotic lesions and showed a trend toward fewer with acute inflammatory changes. VCZ at 40 mg/kg was efficacious in quail with experimental pulmonary aspergillosis.
KW - Antifungal
KW - Aspergillosis
KW - Avian
KW - Fungal infection
KW - Voriconazole
UR - http://www.scopus.com/inward/record.url?scp=76649134835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76649134835&partnerID=8YFLogxK
U2 - 10.3109/13693780903008821
DO - 10.3109/13693780903008821
M3 - Article
VL - 48
SP - 234
EP - 244
JO - Medical Mycology
JF - Medical Mycology
SN - 1369-3786
IS - 2
ER -