Efficacy of voriconazole in Japanese quail (Coturnix japonica) experimentally infected with Aspergillus fumigatus

Lisa A Tell, Karl V. Clemons, Yvonne Kline, Leslie Woods, Philip H Kass, Marife Martinez, David A. Stevens

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Aspergillus fumigatus causes disease in birds. Our objectives were to determine pharmacokinetic parameters and evaluate efficacy of voriconazole (VCZ) in a novel experimental quail model. After a single oral VCZ dose of 20 or 40 mg/kg, plasma concentrations peaked 2 h postdose (5.8 and 6.9 μg/ml) and remained above 0.5 μg/ml for 4 and 12 h postdose, respectively. For the efficacy study, ten-day-old Japanese quail (n 60) were infected intratracheally with A. fumigatus conidia. Daily oral VCZ at 20 or 40 mg/kg was initiated 24 h postinfection (PI); infected diluent-treated birds were given de-ionized water orally. Preassigned birds were euthanized on days 5 or 10 PI. VCZ at 40 mg/kg prolonged survival compared to 20 mg/kg or diluent-treatment (P < 0.05) and lungs from birds given VCZ at 40 mg/kg had fewer colony forming units (CFU) than diluent-treated (P =0.03). At day 10 PI, birds treated with VCZ at 20 mg/kg had significantly fewer fungi in the lungs as demonstrated by methenamine silver stain (P =0.017) or immunohistochemistry, as compared to diluent-treated (P =0.034). Histopathologically, VCZ-treated birds did not have necrotic lesions and showed a trend toward fewer with acute inflammatory changes. VCZ at 40 mg/kg was efficacious in quail with experimental pulmonary aspergillosis.

Original languageEnglish (US)
Pages (from-to)234-244
Number of pages11
JournalMedical Mycology
Volume48
Issue number2
DOIs
StatePublished - 2010

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Keywords

  • Antifungal
  • Aspergillosis
  • Avian
  • Fungal infection
  • Voriconazole

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases

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