Efficacy and toxicity of a CD22-targeted antibody-saporin conjugate in a xenograft model of non-hodgkin's lymphoma

Jason Kato, Robert T O'Donnell, Mastewal Abuhay, Joseph Tuscano

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Antibody drug conjugates (ADCs) can deliver potent drugs to cancer cells by employing the specificity of monoclonal antibodies (mAbs). ADCs have demonstrated significant anticancer activity and, in2011, brentuximab vedotin has been approved by the FDA for the treatment of Hodgkin's and anaplastic large cell lymphomas. CD22 is an ideal target for ADC against B-cell malignancies because of its lineage-specific expression and rapid internalization upon antibody binding. In this study, we evaluated the anti-CD22 mAb HB22.7 as a vehicle for the targeted delivery of the potent toxin saporin (SAP ). In vitro, HB22.7-SAP was cytotoxic against a panel of non-Hodgkin's lymphoma (NHL) cell lines representing the most common types of the disease. Moreover, in a xenograft model of NHL, HB22.7-SAP significantly inhibited the growth of established lesions and completely prevented tumor development when treatment was initiated within 24 h from tumor-cell inoculation. HB22.7-SAP had no significant in vivo toxicity. In conclusion, HB22.7 constitutes a potential platform for CD22-targeted ADCs.

Original languageEnglish (US)
Pages (from-to)1469-1475
Number of pages7
JournalOncoImmunology
Volume1
Issue number9
DOIs
StatePublished - 2012

Keywords

  • Antibody drug conjugate
  • CD22
  • HB22.7
  • Non-hodgkin's lymphoma
  • Saporin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Oncology
  • Immunology

Fingerprint Dive into the research topics of 'Efficacy and toxicity of a CD22-targeted antibody-saporin conjugate in a xenograft model of non-hodgkin's lymphoma'. Together they form a unique fingerprint.

  • Cite this