Efficacies of inocula on the startup of anaerobic reactors treating dairy manure under stirred and unstirred conditions

Pramod Pandey, Pius M. Ndegwa, Michelle L. Soupir, J. Richard Alldredge, Marvin J. Pitts

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Inocula play an important role in anaerobic reactor startup by balancing the populations of Syntrophobacter and methanogens. Such balances make syntrophic metabolism thermodynamically feasible in anaerobic digestion. In this study, the effect of inocula on the performance of dairy manure digestion was investigated by analyzing the change in volatile fatty acids (VFA), total solids (TS), volatile solids (VS), specific biogas production (SGPR), and specific methane production (SPMP) as well as scanning and transmission electron micrographs. The study was performed at four treatments. Treatment one was granular sludge (GM); treatment two was non-granular sludge (SM); treatment three was mixed culture from an anaerobic lagoon (LM); while the fourth treatment (the control denoted MM) did not receive any exogenous inocula. In addition, stirred and unstirred conditions were maintained in the reactors to determine their effect on reactor startup. Performance ranking based on the SGPR and SPMP of treatments (in descending order) was: GM, SM, LM and MM under stirred conditions. Under unstirred conditions, performance ranking (also in descending order) was: SM, GM, LM, and MM. Results of the examination of microcolonies in the granular, non-granular sludge, and dairy manure suggest that syntrophic juxtaposition of methanogens and Syntrophobacter in granular inoculum was common while it was less visible in non-granular sludge, and completely absent in dairy manure.

Original languageEnglish (US)
Pages (from-to)2705-2720
Number of pages16
JournalBiomass and Bioenergy
Volume35
Issue number7
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

Fingerprint

Plant startup
dairy manure
Dairies
Manures
sludge
Syntrophobacter
manure
Reactor startup
inoculum
Methanogens
methanogens
Biogas
biogas
methane production
ranking
Methane
methane
Volatile fatty acids
Anaerobic digestion
anaerobic digestion

Keywords

  • Anaerobic digestion
  • Dairy manure
  • Inocula
  • Methanogens
  • Mixing
  • Syntrophobacter

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

Cite this

Efficacies of inocula on the startup of anaerobic reactors treating dairy manure under stirred and unstirred conditions. / Pandey, Pramod; Ndegwa, Pius M.; Soupir, Michelle L.; Alldredge, J. Richard; Pitts, Marvin J.

In: Biomass and Bioenergy, Vol. 35, No. 7, 01.07.2011, p. 2705-2720.

Research output: Contribution to journalArticle

Pandey, Pramod ; Ndegwa, Pius M. ; Soupir, Michelle L. ; Alldredge, J. Richard ; Pitts, Marvin J. / Efficacies of inocula on the startup of anaerobic reactors treating dairy manure under stirred and unstirred conditions. In: Biomass and Bioenergy. 2011 ; Vol. 35, No. 7. pp. 2705-2720.
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