Abstract
Inocula play an important role in anaerobic reactor startup by balancing the populations of Syntrophobacter and methanogens. Such balances make syntrophic metabolism thermodynamically feasible in anaerobic digestion. In this study, the effect of inocula on the performance of dairy manure digestion was investigated by analyzing the change in volatile fatty acids (VFA), total solids (TS), volatile solids (VS), specific biogas production (SGPR), and specific methane production (SPMP) as well as scanning and transmission electron micrographs. The study was performed at four treatments. Treatment one was granular sludge (GM); treatment two was non-granular sludge (SM); treatment three was mixed culture from an anaerobic lagoon (LM); while the fourth treatment (the control denoted MM) did not receive any exogenous inocula. In addition, stirred and unstirred conditions were maintained in the reactors to determine their effect on reactor startup. Performance ranking based on the SGPR and SPMP of treatments (in descending order) was: GM, SM, LM and MM under stirred conditions. Under unstirred conditions, performance ranking (also in descending order) was: SM, GM, LM, and MM. Results of the examination of microcolonies in the granular, non-granular sludge, and dairy manure suggest that syntrophic juxtaposition of methanogens and Syntrophobacter in granular inoculum was common while it was less visible in non-granular sludge, and completely absent in dairy manure.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2705-2720 |
| Number of pages | 16 |
| Journal | Biomass and Bioenergy |
| Volume | 35 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2011 |
| Externally published | Yes |
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Keywords
- Anaerobic digestion
- Dairy manure
- Inocula
- Methanogens
- Mixing
- Syntrophobacter
ASJC Scopus subject areas
- Forestry
- Renewable Energy, Sustainability and the Environment
- Agronomy and Crop Science
- Waste Management and Disposal
Cite this
Efficacies of inocula on the startup of anaerobic reactors treating dairy manure under stirred and unstirred conditions. / Pandey, Pramod; Ndegwa, Pius M.; Soupir, Michelle L.; Alldredge, J. Richard; Pitts, Marvin J.
In: Biomass and Bioenergy, Vol. 35, No. 7, 01.07.2011, p. 2705-2720.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacies of inocula on the startup of anaerobic reactors treating dairy manure under stirred and unstirred conditions
AU - Pandey, Pramod
AU - Ndegwa, Pius M.
AU - Soupir, Michelle L.
AU - Alldredge, J. Richard
AU - Pitts, Marvin J.
PY - 2011/7/1
Y1 - 2011/7/1
N2 - Inocula play an important role in anaerobic reactor startup by balancing the populations of Syntrophobacter and methanogens. Such balances make syntrophic metabolism thermodynamically feasible in anaerobic digestion. In this study, the effect of inocula on the performance of dairy manure digestion was investigated by analyzing the change in volatile fatty acids (VFA), total solids (TS), volatile solids (VS), specific biogas production (SGPR), and specific methane production (SPMP) as well as scanning and transmission electron micrographs. The study was performed at four treatments. Treatment one was granular sludge (GM); treatment two was non-granular sludge (SM); treatment three was mixed culture from an anaerobic lagoon (LM); while the fourth treatment (the control denoted MM) did not receive any exogenous inocula. In addition, stirred and unstirred conditions were maintained in the reactors to determine their effect on reactor startup. Performance ranking based on the SGPR and SPMP of treatments (in descending order) was: GM, SM, LM and MM under stirred conditions. Under unstirred conditions, performance ranking (also in descending order) was: SM, GM, LM, and MM. Results of the examination of microcolonies in the granular, non-granular sludge, and dairy manure suggest that syntrophic juxtaposition of methanogens and Syntrophobacter in granular inoculum was common while it was less visible in non-granular sludge, and completely absent in dairy manure.
AB - Inocula play an important role in anaerobic reactor startup by balancing the populations of Syntrophobacter and methanogens. Such balances make syntrophic metabolism thermodynamically feasible in anaerobic digestion. In this study, the effect of inocula on the performance of dairy manure digestion was investigated by analyzing the change in volatile fatty acids (VFA), total solids (TS), volatile solids (VS), specific biogas production (SGPR), and specific methane production (SPMP) as well as scanning and transmission electron micrographs. The study was performed at four treatments. Treatment one was granular sludge (GM); treatment two was non-granular sludge (SM); treatment three was mixed culture from an anaerobic lagoon (LM); while the fourth treatment (the control denoted MM) did not receive any exogenous inocula. In addition, stirred and unstirred conditions were maintained in the reactors to determine their effect on reactor startup. Performance ranking based on the SGPR and SPMP of treatments (in descending order) was: GM, SM, LM and MM under stirred conditions. Under unstirred conditions, performance ranking (also in descending order) was: SM, GM, LM, and MM. Results of the examination of microcolonies in the granular, non-granular sludge, and dairy manure suggest that syntrophic juxtaposition of methanogens and Syntrophobacter in granular inoculum was common while it was less visible in non-granular sludge, and completely absent in dairy manure.
KW - Anaerobic digestion
KW - Dairy manure
KW - Inocula
KW - Methanogens
KW - Mixing
KW - Syntrophobacter
UR - http://www.scopus.com/inward/record.url?scp=79958136282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79958136282&partnerID=8YFLogxK
U2 - 10.1016/j.biombioe.2011.03.017
DO - 10.1016/j.biombioe.2011.03.017
M3 - Article
AN - SCOPUS:79958136282
VL - 35
SP - 2705
EP - 2720
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
SN - 0961-9534
IS - 7
ER -