Effects of TRPA1 agonists mustard oil and cinnamaldehyde on lumbar spinal wide-dynamic range neuronal responses to innocuous and noxious cutaneous stimuli in rats

Austin W. Merrill, Jason M. Cuellar, Justin H. Judd, Mirela Iodi Carstens, Earl Carstens

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Mustard oil [allyl isothiocyanate (AITC)] and cinnamaldehyde (CA), agonists of the ion channel TRPA1 expressed in sensory neurons, elicit a burning sensation and heat hyperalgesia. We tested whether these phenomena are reflected in the responses of lumbar spinal wide-dynamic range (WDR) neurons recorded in pentobarbital-anesthetized rats. Responses to electrical and graded mechanical and noxious thermal stimulation were tested before and after cutaneous application of AITC or CA. Repetitive application of AITC initially increased the firing rate of 52% of units followed by rapid desensitization that persisted when AITC was reapplied 30 min later. Responses to noxious thermal, but not mechanical, stimuli were significantly enhanced irrespective of whether the neuron was directly activated by AITC. Windup elicited by percutaneous or sciatic nerve electrical stimulation was significantly reduced post-AITC. These results indicate that AITC produced central inhibition and peripheral sensitization of heat nociceptors. CA did not directly excite WDR neurons, and significantly enhanced responses to noxious heat while not affecting windup or responses to skin cooling or mechanical stimulation, indicating a peripheral sensitization of heat nociceptors.

Original languageEnglish (US)
Pages (from-to)415-425
Number of pages11
JournalJournal of Neurophysiology
Volume99
Issue number2
DOIs
StatePublished - Feb 2008

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Hot Temperature
Skin
Nociceptors
Neurons
Hyperalgesia
Sensory Receptor Cells
Sciatic Nerve
Pentobarbital
mustard oil
cinnamic aldehyde
allyl isothiocyanate
Ion Channels
Electric Stimulation

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Effects of TRPA1 agonists mustard oil and cinnamaldehyde on lumbar spinal wide-dynamic range neuronal responses to innocuous and noxious cutaneous stimuli in rats. / Merrill, Austin W.; Cuellar, Jason M.; Judd, Justin H.; Carstens, Mirela Iodi; Carstens, Earl.

In: Journal of Neurophysiology, Vol. 99, No. 2, 02.2008, p. 415-425.

Research output: Contribution to journalArticle

Merrill, Austin W. ; Cuellar, Jason M. ; Judd, Justin H. ; Carstens, Mirela Iodi ; Carstens, Earl. / Effects of TRPA1 agonists mustard oil and cinnamaldehyde on lumbar spinal wide-dynamic range neuronal responses to innocuous and noxious cutaneous stimuli in rats. In: Journal of Neurophysiology. 2008 ; Vol. 99, No. 2. pp. 415-425.
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