Abstract
Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed to survive posttrauma for periods of 5 min, 3 h, and 24 h. The effect of TBI on binding to forebrain opiate receptors was assessed using quantitative receptor autoradiography, and compared to a sham control group. Binding of [3H]DAGO to mu receptors in neocortex and the CA1 pyramidal layer of the hippocampus was significantly decreased in the 24-h group (p<0.05). [3H]Bremazocine binding to kappa receptors was unchanged at 5 min and 24 h, but showed large decreases 3 h after TBI in the CA1 pyramidal layer (65%, p<0.05) and dentate gyrus (43%, p<0.05). Levels of delta binding (measured with [3H]DSLET) and lambda binding (measured with [3H]naloxone) were unaffected by TBI. These data support previous suggestions of a role for endogenous opioids in TBI, and provide further evidence that mu and kappa opioid receptor subtypes in neocortex and hippocampus may have different functions in TBI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-107 |
| Number of pages | 13 |
| Journal | Molecular and Chemical Neuropathology |
| Volume | 16 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Feb 1992 |
| Externally published | Yes |
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Keywords
- dentate gyrus
- endogenous opioids
- excitotoxicity
- hippocampus
- kappa opiate receptors
- mu opiate receptors
- Traumatic brain injury
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Molecular Biology
Cite this
Effects of traumatic brain injury in rats on binding to forebrain opiate receptor subtypes. / Perry, David C.; Lyeth, Bruce G; Miller, Leonard P.; Getz, Rena L.; Jenkins, Larry W.; Hayes, Ronald L.
In: Molecular and Chemical Neuropathology, Vol. 16, No. 1-2, 02.1992, p. 95-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of traumatic brain injury in rats on binding to forebrain opiate receptor subtypes
AU - Perry, David C.
AU - Lyeth, Bruce G
AU - Miller, Leonard P.
AU - Getz, Rena L.
AU - Jenkins, Larry W.
AU - Hayes, Ronald L.
PY - 1992/2
Y1 - 1992/2
N2 - Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed to survive posttrauma for periods of 5 min, 3 h, and 24 h. The effect of TBI on binding to forebrain opiate receptors was assessed using quantitative receptor autoradiography, and compared to a sham control group. Binding of [3H]DAGO to mu receptors in neocortex and the CA1 pyramidal layer of the hippocampus was significantly decreased in the 24-h group (p<0.05). [3H]Bremazocine binding to kappa receptors was unchanged at 5 min and 24 h, but showed large decreases 3 h after TBI in the CA1 pyramidal layer (65%, p<0.05) and dentate gyrus (43%, p<0.05). Levels of delta binding (measured with [3H]DSLET) and lambda binding (measured with [3H]naloxone) were unaffected by TBI. These data support previous suggestions of a role for endogenous opioids in TBI, and provide further evidence that mu and kappa opioid receptor subtypes in neocortex and hippocampus may have different functions in TBI.
AB - Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed to survive posttrauma for periods of 5 min, 3 h, and 24 h. The effect of TBI on binding to forebrain opiate receptors was assessed using quantitative receptor autoradiography, and compared to a sham control group. Binding of [3H]DAGO to mu receptors in neocortex and the CA1 pyramidal layer of the hippocampus was significantly decreased in the 24-h group (p<0.05). [3H]Bremazocine binding to kappa receptors was unchanged at 5 min and 24 h, but showed large decreases 3 h after TBI in the CA1 pyramidal layer (65%, p<0.05) and dentate gyrus (43%, p<0.05). Levels of delta binding (measured with [3H]DSLET) and lambda binding (measured with [3H]naloxone) were unaffected by TBI. These data support previous suggestions of a role for endogenous opioids in TBI, and provide further evidence that mu and kappa opioid receptor subtypes in neocortex and hippocampus may have different functions in TBI.
KW - dentate gyrus
KW - endogenous opioids
KW - excitotoxicity
KW - hippocampus
KW - kappa opiate receptors
KW - mu opiate receptors
KW - Traumatic brain injury
UR - http://www.scopus.com/inward/record.url?scp=0026687137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026687137&partnerID=8YFLogxK
U2 - 10.1007/BF03159963
DO - 10.1007/BF03159963
M3 - Article
C2 - 1325802
AN - SCOPUS:0026687137
VL - 16
SP - 95
EP - 107
JO - Journal of Molecular Neuroscience
JF - Journal of Molecular Neuroscience
SN - 0895-8696
IS - 1-2
ER -