Effects of traumatic brain injury in rats on binding to forebrain opiate receptor subtypes

David C. Perry, Bruce G Lyeth, Leonard P. Miller, Rena L. Getz, Larry W. Jenkins, Ronald L. Hayes

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed to survive posttrauma for periods of 5 min, 3 h, and 24 h. The effect of TBI on binding to forebrain opiate receptors was assessed using quantitative receptor autoradiography, and compared to a sham control group. Binding of [3H]DAGO to mu receptors in neocortex and the CA1 pyramidal layer of the hippocampus was significantly decreased in the 24-h group (p<0.05). [3H]Bremazocine binding to kappa receptors was unchanged at 5 min and 24 h, but showed large decreases 3 h after TBI in the CA1 pyramidal layer (65%, p<0.05) and dentate gyrus (43%, p<0.05). Levels of delta binding (measured with [3H]DSLET) and lambda binding (measured with [3H]naloxone) were unaffected by TBI. These data support previous suggestions of a role for endogenous opioids in TBI, and provide further evidence that mu and kappa opioid receptor subtypes in neocortex and hippocampus may have different functions in TBI.

Original languageEnglish (US)
Pages (from-to)95-107
Number of pages13
JournalMolecular and Chemical Neuropathology
Volume16
Issue number1-2
DOIs
StatePublished - Feb 1992
Externally publishedYes

Fingerprint

Opioid Receptors
Prosencephalon
kappa Opioid Receptor
enkephalin, Ser(2), Leu(5), Thr(6)-
mu Opioid Receptor
Neocortex
Hippocampus
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Percussion
Dentate Gyrus
Naloxone
Autoradiography
Opioid Analgesics
Sprague Dawley Rats
Traumatic Brain Injury
Control Groups

Keywords

  • dentate gyrus
  • endogenous opioids
  • excitotoxicity
  • hippocampus
  • kappa opiate receptors
  • mu opiate receptors
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

Cite this

Effects of traumatic brain injury in rats on binding to forebrain opiate receptor subtypes. / Perry, David C.; Lyeth, Bruce G; Miller, Leonard P.; Getz, Rena L.; Jenkins, Larry W.; Hayes, Ronald L.

In: Molecular and Chemical Neuropathology, Vol. 16, No. 1-2, 02.1992, p. 95-107.

Research output: Contribution to journalArticle

Perry, David C. ; Lyeth, Bruce G ; Miller, Leonard P. ; Getz, Rena L. ; Jenkins, Larry W. ; Hayes, Ronald L. / Effects of traumatic brain injury in rats on binding to forebrain opiate receptor subtypes. In: Molecular and Chemical Neuropathology. 1992 ; Vol. 16, No. 1-2. pp. 95-107.
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