Effects of tissue preincubation and hypoxia on CA3 hippocampal neurons in the in vitro slice preparation

Anna K. Davis, Damir Janigro, Philip A Schwartzkroin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Using the in vitro hippocampal slice preparation, we studied the electrophysiological properties of pyramidal cells in tissue that was 'preincubated' (2-6 h in a large, static volume of oxygenated bathing medium) before being placed in an interface chamber for study. Striking differences were found in 'preincubated' vs 'non-preincubated' CA3 cells. The preincubated cells had more negative resting potentials, higher input resistance, lower threshold for stimulus-evoked burst discharge and larger hyperpolarizing afterpotentials. Cells in the preincubated CA3 region were also more likely to show spontaneous synchronized burst discharge, but were relatively resistant to hypoxia-induced spreading depression. CA1 cells were less dramatically affected by preincubation, showing little difference from their non-preincubated counterparts. Possible mechanisms involved in the CA3 preincubation effect, including glial buffering alterations and changes in Na+,K+-ATPase activity, are discussed.

Original languageEnglish (US)
Pages (from-to)44-53
Number of pages10
JournalBrain Research
Volume370
Issue number1
DOIs
StatePublished - Apr 2 1986
Externally publishedYes

Keywords

  • CA3 neuron
  • hippocampus
  • hypoxia
  • incubation
  • slice preparation

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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