Effects of tissue optical properties on time-resolved fluorescence measurements from brain tumors: An experimental and computational study

Pramod V. Butte, Karthik Vishwanath, Brian Pikul, Mary Ann Mycek, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Time-Resolved Laser-Induced Fluorescence Spectroscopy (tr-LIFS) offers the potential for intra-operative diagnosis of primary brain tumors. However, both the intrinsic properties of endogenous fluorophores and the optical properties of brain tissue could affect the fluorescence measurements from brain. Scattering has been demonstrated to increase, for instance, detected lifetimes by 10-20% in media less scattering than the brain. The overall goal of this study is to investigate experimentally and computationally how optical properties of distinct types of brain tissue (normal porcine white and gray matter) affect the propagation of the excitation pulse and fluorescent transients and the detected fluorescence lifetime. A time-domain tr-LIFS apparatus (fast digitizer and gated detection) was employed to measure the propagation of ultra-short pulsed light through brain specimens (1-2.5-mm source-detector separation; 0.100-mm increment). A Monte Carlo model for semi-infinite turbid media was used to simulate time-resolved light propagation for arbitrary source-detector fiber geometries and optical fiber specifications; and to record spatially- and temporally resolved information. We determined a good correlation between experimental and computational results. Our findings provide means for quantification of time-resolved fluorescence spectra from healthy and diseased brain tissue.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsB. Chance, R.R. Alfano, B.J. Tromberg, M. Tamura, E.M. Sevick-Muraca
Pages600-608
Number of pages9
Volume4955
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V - San Jose, CA, United States
Duration: Jan 26 2003Jan 29 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V
CountryUnited States
CitySan Jose, CA
Period1/26/031/29/03

Keywords

  • Brain
  • Lifetime fluorescence
  • Optical coefficients
  • Optical properties
  • Pulse width distortion

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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  • Cite this

    Butte, P. V., Vishwanath, K., Pikul, B., Mycek, M. A., & Marcu, L. (2003). Effects of tissue optical properties on time-resolved fluorescence measurements from brain tumors: An experimental and computational study. In B. Chance, R. R. Alfano, B. J. Tromberg, M. Tamura, & E. M. Sevick-Muraca (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4955, pp. 600-608) https://doi.org/10.1117/12.478192