Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes

Eugene J. Koay, Gidon Ofek, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The responses of articular chondrocytes to physicochemical stimuli are intimately linked to processes that can lead to both degenerative and regenerative processes. Toward understanding this link, we examined the biomechanical behavior of single chondrocytes in response to growth factors (IGF-I and TGF-β1) and a range of compressive strains. The results indicate that the growth factors alter the biomechanics of the cells in terms of their stiffness coefficient (∼two-fold increase over control) and compressibility, as measured by an apparent Poisson's ratio (∼two-fold increase over control also). Interestingly, the compressibility decreased significantly with respect to the applied strain. Moreover, we have again detected a critical strain threshold in chondrocytes at ∼30% strain in all treatments. Overall, these findings demonstrate that cellular biomechanics change in response to both biochemical and biomechanical perturbations. Understanding the underlying biomechanics of chondrocytes in response to such stimuli may be useful in understanding various aspects of cartilage, including the study of osteoarthritis and the development of tissue-engineering strategies.

Original languageEnglish (US)
Pages (from-to)1044-1052
Number of pages9
JournalJournal of Biomechanics
Volume41
Issue number5
DOIs
StatePublished - 2008
Externally publishedYes

Keywords

  • Cartilage
  • Chondrocyte
  • Compression
  • Mechanobiology
  • Poisson's ratio

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Fingerprint Dive into the research topics of 'Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes'. Together they form a unique fingerprint.

  • Cite this