Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes

Eugene J. Koay, Gidon Ofek, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The responses of articular chondrocytes to physicochemical stimuli are intimately linked to processes that can lead to both degenerative and regenerative processes. Toward understanding this link, we examined the biomechanical behavior of single chondrocytes in response to growth factors (IGF-I and TGF-β1) and a range of compressive strains. The results indicate that the growth factors alter the biomechanics of the cells in terms of their stiffness coefficient (∼two-fold increase over control) and compressibility, as measured by an apparent Poisson's ratio (∼two-fold increase over control also). Interestingly, the compressibility decreased significantly with respect to the applied strain. Moreover, we have again detected a critical strain threshold in chondrocytes at ∼30% strain in all treatments. Overall, these findings demonstrate that cellular biomechanics change in response to both biochemical and biomechanical perturbations. Understanding the underlying biomechanics of chondrocytes in response to such stimuli may be useful in understanding various aspects of cartilage, including the study of osteoarthritis and the development of tissue-engineering strategies.

Original languageEnglish (US)
Pages (from-to)1044-1052
Number of pages9
JournalJournal of Biomechanics
Volume41
Issue number5
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Biomechanics
Chondrocytes
Insulin-Like Growth Factor I
Compressibility
Biomechanical Phenomena
Recovery
Intercellular Signaling Peptides and Proteins
Cartilage
Poisson ratio
Tissue Engineering
Tissue engineering
Osteoarthritis
Joints
Stiffness

Keywords

  • Cartilage
  • Chondrocyte
  • Compression
  • Mechanobiology
  • Poisson's ratio

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes. / Koay, Eugene J.; Ofek, Gidon; Athanasiou, Kyriacos A.

In: Journal of Biomechanics, Vol. 41, No. 5, 2008, p. 1044-1052.

Research output: Contribution to journalArticle

Koay, Eugene J. ; Ofek, Gidon ; Athanasiou, Kyriacos A. / Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes. In: Journal of Biomechanics. 2008 ; Vol. 41, No. 5. pp. 1044-1052.
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