Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs

Benjamin D. Elder, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The objective of this study was to determine the effects of temporal hydrostatic pressure (HP) on the properties of scaffoldless bovine articular cartilage constructs. The study was organized in three phases: First, a suitable control for HP application was identified. Second, 10 MPa static HP was applied at three different timepoints (6-10 days, 10-14 days, and 14-18 days) to identify a window in construct development when HP application would be most beneficial. Third, the temporal effects of 10-14-day static HP application, as determined in phase II, were assessed at 2, 4, and 8 weeks. Compressive and tensile mechanical properties, GAG and collagen content, histology for GAG and collagen, and immunohistochemistry for collagen types I and II were assessed. When a culture control identified in phase I was used in phase II, HP application from 10 to 14 days resulted in a significant 1.4-fold increase in aggregate modulus, accompanied by an increase in GAG content, while HP application at all timepoints enhanced tensile properties and collagen content. In phase III, HP had an immediate effect on GAG content, collagen content, and compressive stiffness, while there was a delayed increase in tensile stiffness. The enhanced tensile stiffness was still present at 8 weeks. For the first time, this study examined the immediate and long-term effects of HP on biomechanical properties, and demonstrated that HP has an optimal application time in construct development. These findings are exciting as HP stimulation allowed for the formation of robust tissue-engineered cartilage; for example, 10 MPa static HP resulted in an aggregate modulus of 273 ± 123 kPa, a Young's modulus of 1.6 ± 0.4 MPa, a GAG/wet weight of 6.1 ± 1.4%, and a collagen/wet weight of 10.6 ± 2.4% at 4 weeks.

Original languageEnglish (US)
Pages (from-to)1151-1158
Number of pages8
JournalTissue Engineering - Part A
Volume15
Issue number5
DOIs
StatePublished - May 1 2009
Externally publishedYes

Fingerprint

Hydrostatic Pressure
Cartilage
Articular Cartilage
Hydrostatic pressure
Tissue
Collagen
Stiffness
Weights and Measures
Time and motion study
Histology
Time and Motion Studies
Collagen Type II
Elastic Modulus
Collagen Type I
Tensile properties
Elastic moduli
Immunohistochemistry

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs. / Elder, Benjamin D.; Athanasiou, Kyriacos A.

In: Tissue Engineering - Part A, Vol. 15, No. 5, 01.05.2009, p. 1151-1158.

Research output: Contribution to journalArticle

Elder, Benjamin D. ; Athanasiou, Kyriacos A. / Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 5. pp. 1151-1158.
@article{bccabc0612f34b1fb7be18cb8979c3cd,
title = "Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs",
abstract = "The objective of this study was to determine the effects of temporal hydrostatic pressure (HP) on the properties of scaffoldless bovine articular cartilage constructs. The study was organized in three phases: First, a suitable control for HP application was identified. Second, 10 MPa static HP was applied at three different timepoints (6-10 days, 10-14 days, and 14-18 days) to identify a window in construct development when HP application would be most beneficial. Third, the temporal effects of 10-14-day static HP application, as determined in phase II, were assessed at 2, 4, and 8 weeks. Compressive and tensile mechanical properties, GAG and collagen content, histology for GAG and collagen, and immunohistochemistry for collagen types I and II were assessed. When a culture control identified in phase I was used in phase II, HP application from 10 to 14 days resulted in a significant 1.4-fold increase in aggregate modulus, accompanied by an increase in GAG content, while HP application at all timepoints enhanced tensile properties and collagen content. In phase III, HP had an immediate effect on GAG content, collagen content, and compressive stiffness, while there was a delayed increase in tensile stiffness. The enhanced tensile stiffness was still present at 8 weeks. For the first time, this study examined the immediate and long-term effects of HP on biomechanical properties, and demonstrated that HP has an optimal application time in construct development. These findings are exciting as HP stimulation allowed for the formation of robust tissue-engineered cartilage; for example, 10 MPa static HP resulted in an aggregate modulus of 273 ± 123 kPa, a Young's modulus of 1.6 ± 0.4 MPa, a GAG/wet weight of 6.1 ± 1.4{\%}, and a collagen/wet weight of 10.6 ± 2.4{\%} at 4 weeks.",
author = "Elder, {Benjamin D.} and Athanasiou, {Kyriacos A.}",
year = "2009",
month = "5",
day = "1",
doi = "10.1089/ten.tea.2008.0200",
language = "English (US)",
volume = "15",
pages = "1151--1158",
journal = "Tissue Engineering - Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs

AU - Elder, Benjamin D.

AU - Athanasiou, Kyriacos A.

PY - 2009/5/1

Y1 - 2009/5/1

N2 - The objective of this study was to determine the effects of temporal hydrostatic pressure (HP) on the properties of scaffoldless bovine articular cartilage constructs. The study was organized in three phases: First, a suitable control for HP application was identified. Second, 10 MPa static HP was applied at three different timepoints (6-10 days, 10-14 days, and 14-18 days) to identify a window in construct development when HP application would be most beneficial. Third, the temporal effects of 10-14-day static HP application, as determined in phase II, were assessed at 2, 4, and 8 weeks. Compressive and tensile mechanical properties, GAG and collagen content, histology for GAG and collagen, and immunohistochemistry for collagen types I and II were assessed. When a culture control identified in phase I was used in phase II, HP application from 10 to 14 days resulted in a significant 1.4-fold increase in aggregate modulus, accompanied by an increase in GAG content, while HP application at all timepoints enhanced tensile properties and collagen content. In phase III, HP had an immediate effect on GAG content, collagen content, and compressive stiffness, while there was a delayed increase in tensile stiffness. The enhanced tensile stiffness was still present at 8 weeks. For the first time, this study examined the immediate and long-term effects of HP on biomechanical properties, and demonstrated that HP has an optimal application time in construct development. These findings are exciting as HP stimulation allowed for the formation of robust tissue-engineered cartilage; for example, 10 MPa static HP resulted in an aggregate modulus of 273 ± 123 kPa, a Young's modulus of 1.6 ± 0.4 MPa, a GAG/wet weight of 6.1 ± 1.4%, and a collagen/wet weight of 10.6 ± 2.4% at 4 weeks.

AB - The objective of this study was to determine the effects of temporal hydrostatic pressure (HP) on the properties of scaffoldless bovine articular cartilage constructs. The study was organized in three phases: First, a suitable control for HP application was identified. Second, 10 MPa static HP was applied at three different timepoints (6-10 days, 10-14 days, and 14-18 days) to identify a window in construct development when HP application would be most beneficial. Third, the temporal effects of 10-14-day static HP application, as determined in phase II, were assessed at 2, 4, and 8 weeks. Compressive and tensile mechanical properties, GAG and collagen content, histology for GAG and collagen, and immunohistochemistry for collagen types I and II were assessed. When a culture control identified in phase I was used in phase II, HP application from 10 to 14 days resulted in a significant 1.4-fold increase in aggregate modulus, accompanied by an increase in GAG content, while HP application at all timepoints enhanced tensile properties and collagen content. In phase III, HP had an immediate effect on GAG content, collagen content, and compressive stiffness, while there was a delayed increase in tensile stiffness. The enhanced tensile stiffness was still present at 8 weeks. For the first time, this study examined the immediate and long-term effects of HP on biomechanical properties, and demonstrated that HP has an optimal application time in construct development. These findings are exciting as HP stimulation allowed for the formation of robust tissue-engineered cartilage; for example, 10 MPa static HP resulted in an aggregate modulus of 273 ± 123 kPa, a Young's modulus of 1.6 ± 0.4 MPa, a GAG/wet weight of 6.1 ± 1.4%, and a collagen/wet weight of 10.6 ± 2.4% at 4 weeks.

UR - http://www.scopus.com/inward/record.url?scp=66249102002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66249102002&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2008.0200

DO - 10.1089/ten.tea.2008.0200

M3 - Article

C2 - 18831685

AN - SCOPUS:66249102002

VL - 15

SP - 1151

EP - 1158

JO - Tissue Engineering - Part A

JF - Tissue Engineering - Part A

SN - 1937-3341

IS - 5

ER -