Effects of temperature on infectivity and of commercial freezing on survival of the North American strain of viral hemorrhagic septicemia virus (VHSV)

Kristen D. Arkush, Holly L. Mendonca, Anne M. McBride, Susan Yun, Terry S. McDowell, Ronald Hedrick

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Temperature affected the growth of the North American strain of viral hemorrhagic septicemia virus (VHSV) in experimentally infected cell cultures and in Pacific sardine Sardinops sagax. In addition, commercial freezing significantly reduced the infectivity of VHSV in tissues of experimentally infected sardine. Isolates of VHSV representing the geographic range of North American VHSV replicated in the EPC (Epithelioma papulosum cyprini) cell line at 10, 15 and 20°C, but the more northern isolates from British Columbia, Canada, demonstrated significantly reduced growth at 20°C compared to VHSV from more southern locations (p < 0.001). An injection challenge of Pacific sardine with VHSV from California resulted in 66.7% mortality at a seawater temperature of 13°C compared to 6.7% at 20°C. Commercial blast-freezing of sardine experimentally infected with VHSV reduced median concentrations of virus in the kidney and spleen from 5.25 × 106 to 5.5 × 103 pfu (plaque-forming units) g-1. Decreased growth of the California isolate of VHSV at higher temperatures following experimental infection of the sardine and reduced virus survival following commercial freezing of infected sardine are factors that would lessen the risk of transmission of VHSV through frozen baitfishes.

Original languageEnglish (US)
Pages (from-to)145-151
Number of pages7
JournalDiseases of Aquatic Organisms
Volume69
Issue number2-3
DOIs
StatePublished - Apr 6 2006

Fingerprint

Viral hemorrhagic septicemia virus
infectivity
freezing
virus
pathogenicity
sardines
Sardinops sagax
temperature
Papulosa
baitfish
effect
viruses
British Columbia
spleen
cell culture
seawater
cell lines
kidneys
Canada
injection

Keywords

  • Baitfishes
  • Sardine
  • Temperature
  • VHSV

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Effects of temperature on infectivity and of commercial freezing on survival of the North American strain of viral hemorrhagic septicemia virus (VHSV). / Arkush, Kristen D.; Mendonca, Holly L.; McBride, Anne M.; Yun, Susan; McDowell, Terry S.; Hedrick, Ronald.

In: Diseases of Aquatic Organisms, Vol. 69, No. 2-3, 06.04.2006, p. 145-151.

Research output: Contribution to journalArticle

Arkush, Kristen D. ; Mendonca, Holly L. ; McBride, Anne M. ; Yun, Susan ; McDowell, Terry S. ; Hedrick, Ronald. / Effects of temperature on infectivity and of commercial freezing on survival of the North American strain of viral hemorrhagic septicemia virus (VHSV). In: Diseases of Aquatic Organisms. 2006 ; Vol. 69, No. 2-3. pp. 145-151.
@article{f71ac889582148eb8fb63f4a5d5897f4,
title = "Effects of temperature on infectivity and of commercial freezing on survival of the North American strain of viral hemorrhagic septicemia virus (VHSV)",
abstract = "Temperature affected the growth of the North American strain of viral hemorrhagic septicemia virus (VHSV) in experimentally infected cell cultures and in Pacific sardine Sardinops sagax. In addition, commercial freezing significantly reduced the infectivity of VHSV in tissues of experimentally infected sardine. Isolates of VHSV representing the geographic range of North American VHSV replicated in the EPC (Epithelioma papulosum cyprini) cell line at 10, 15 and 20°C, but the more northern isolates from British Columbia, Canada, demonstrated significantly reduced growth at 20°C compared to VHSV from more southern locations (p < 0.001). An injection challenge of Pacific sardine with VHSV from California resulted in 66.7{\%} mortality at a seawater temperature of 13°C compared to 6.7{\%} at 20°C. Commercial blast-freezing of sardine experimentally infected with VHSV reduced median concentrations of virus in the kidney and spleen from 5.25 × 106 to 5.5 × 103 pfu (plaque-forming units) g-1. Decreased growth of the California isolate of VHSV at higher temperatures following experimental infection of the sardine and reduced virus survival following commercial freezing of infected sardine are factors that would lessen the risk of transmission of VHSV through frozen baitfishes.",
keywords = "Baitfishes, Sardine, Temperature, VHSV",
author = "Arkush, {Kristen D.} and Mendonca, {Holly L.} and McBride, {Anne M.} and Susan Yun and McDowell, {Terry S.} and Ronald Hedrick",
year = "2006",
month = "4",
day = "6",
doi = "10.3354/dao069145",
language = "English (US)",
volume = "69",
pages = "145--151",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "2-3",

}

TY - JOUR

T1 - Effects of temperature on infectivity and of commercial freezing on survival of the North American strain of viral hemorrhagic septicemia virus (VHSV)

AU - Arkush, Kristen D.

AU - Mendonca, Holly L.

AU - McBride, Anne M.

AU - Yun, Susan

AU - McDowell, Terry S.

AU - Hedrick, Ronald

PY - 2006/4/6

Y1 - 2006/4/6

N2 - Temperature affected the growth of the North American strain of viral hemorrhagic septicemia virus (VHSV) in experimentally infected cell cultures and in Pacific sardine Sardinops sagax. In addition, commercial freezing significantly reduced the infectivity of VHSV in tissues of experimentally infected sardine. Isolates of VHSV representing the geographic range of North American VHSV replicated in the EPC (Epithelioma papulosum cyprini) cell line at 10, 15 and 20°C, but the more northern isolates from British Columbia, Canada, demonstrated significantly reduced growth at 20°C compared to VHSV from more southern locations (p < 0.001). An injection challenge of Pacific sardine with VHSV from California resulted in 66.7% mortality at a seawater temperature of 13°C compared to 6.7% at 20°C. Commercial blast-freezing of sardine experimentally infected with VHSV reduced median concentrations of virus in the kidney and spleen from 5.25 × 106 to 5.5 × 103 pfu (plaque-forming units) g-1. Decreased growth of the California isolate of VHSV at higher temperatures following experimental infection of the sardine and reduced virus survival following commercial freezing of infected sardine are factors that would lessen the risk of transmission of VHSV through frozen baitfishes.

AB - Temperature affected the growth of the North American strain of viral hemorrhagic septicemia virus (VHSV) in experimentally infected cell cultures and in Pacific sardine Sardinops sagax. In addition, commercial freezing significantly reduced the infectivity of VHSV in tissues of experimentally infected sardine. Isolates of VHSV representing the geographic range of North American VHSV replicated in the EPC (Epithelioma papulosum cyprini) cell line at 10, 15 and 20°C, but the more northern isolates from British Columbia, Canada, demonstrated significantly reduced growth at 20°C compared to VHSV from more southern locations (p < 0.001). An injection challenge of Pacific sardine with VHSV from California resulted in 66.7% mortality at a seawater temperature of 13°C compared to 6.7% at 20°C. Commercial blast-freezing of sardine experimentally infected with VHSV reduced median concentrations of virus in the kidney and spleen from 5.25 × 106 to 5.5 × 103 pfu (plaque-forming units) g-1. Decreased growth of the California isolate of VHSV at higher temperatures following experimental infection of the sardine and reduced virus survival following commercial freezing of infected sardine are factors that would lessen the risk of transmission of VHSV through frozen baitfishes.

KW - Baitfishes

KW - Sardine

KW - Temperature

KW - VHSV

UR - http://www.scopus.com/inward/record.url?scp=33646526518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646526518&partnerID=8YFLogxK

U2 - 10.3354/dao069145

DO - 10.3354/dao069145

M3 - Article

C2 - 16724558

AN - SCOPUS:33646526518

VL - 69

SP - 145

EP - 151

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 2-3

ER -