Effects of temperature alterations on population and cellular activities in hippocampal slices from mature and immature rabbit

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Abstract

Effects of temperature on population spike and cellular activities have been assessed in the CA1 region of hippocampal slices from mature and immature rabbit. In field potential recordings, population spike amplitude was maximal at near 30 °C for both mature and immature tissue, and fell off as temperature was either raised (to a maximum of 44 °C) or lowered (to a minimum of 20 °C). With cooling below 30 °C, population spikes decreased in amplitude and became broader; stimuli always elicited some response, and changes due to cooling were reversible. With increases in temperature, however, irreversible decrease and/or loss of population spikes occurred when tissue was warmed beyond 43 °C. Input-output curves established for mature and immature slices indicated that, at all temperatures, population spike amplitude grew more rapidly with small increases in stimulus intensity in immature slices as compared to mature slices. Intracellular recordings were made from CA1 pyramidal cells in mature and immature hippocampal slices. For both mature and immature tissues, moderate warming (to 40 °C) produced membrane hyperpolarizations in many cells, especially in the mature hippocampus. Increasing temperature beyond 40 °C led to marked depolarizations in a number of cells, a depolarization that was irreversible, particularly in mature neurons. Cooling generally produced a depolarizing shift in membrane potential and an accompanying increase in input resistance; these effects, however, were reversible. Temperature changes in both warming and cooling directions had effects on repetitive firing patterns in both mature and immature neurons. In particular, spike trains elicited by a constant current pulse at a given membrane potential became shorter. The effects of cooling on this cell parameter were reversible, but warming-induced changes were usually permanent. Irreversibility of the warming effects was more pronounced in cells from mature than from immature hippocampus. As reported previously, cooling produced marked spike broadening and changes in synaptic potentials in both mature and immature neurons. These studies confirm previously reported temperature sensitivities of neuronal properties in hippocampal slices. On the basis of these data, and reports from other laboratories, it is clear that relatively small changes in temperature can have rather dramatic effects on properties of single cells and cell populations. Such temperature sensitivity is critical in evaluating data obtained from in vitro slice preparations. These data are also helpful in trying to evaluate the effects of hyperthermia (fever) on neural function.

Original languageEnglish (US)
Pages (from-to)305-316
Number of pages12
JournalBrain Research
Volume475
Issue number2
DOIs
StatePublished - Dec 20 1988
Externally publishedYes

Fingerprint

Rabbits
Temperature
Population
Neurons
Membrane Potentials
Hippocampus
Fever
Hippocampal CA1 Region
Synaptic Potentials
Pyramidal Cells
Cell Count
Membranes

Keywords

  • CA1 pyramidal cell
  • Hippocampal slice
  • Immature nervous system
  • Temperature sensitivity

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Effects of temperature alterations on population and cellular activities in hippocampal slices from mature and immature rabbit. / Shen, Ke fei; Schwartzkroin, Philip A.

In: Brain Research, Vol. 475, No. 2, 20.12.1988, p. 305-316.

Research output: Contribution to journalArticle

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