Effects of substrata on the polarization of bovine endometrial epithelial cells in vitro

Linda Munson, J. Erby Wilkinson, Donald H. Schlafer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Epithelial-cell function requires cellular polarity in which apical membrane surfaces have unique characteristics and cellular organelles are stratified. Physiological investigations of endometrial, epithelial cells would be enhanced greatly by the ability of a method to polarize cells in culture. This study investigates the effects of different substrata on polarization of cultured bovine endometrial epithelial cells. Fetal bovine endometrial epithelial-cell lines were developed from explant outgrowth. Epithelial monolayers were subcultured onto amniotic membranes, Millicell-HA membranes, or Millicell-CM membranes coated with rat-tail collagen, Matrigel, laminin, Vitrogen,or fibronectin. Cultures on these substrata were maintained at the air/liquid interface. Cells grown on either collagen-coated or uncoated Milli-cell membranes also were maintained submerged in medium. Excellent polarized morphology was attained in cultures grown at the air/liquid interface on amniotic membranes and rat-tail collagen-coated membranes. Lectin-binding patterns, to apical membranes of polarized epithelial cell cultures paralleled patterns of binding to bovine endometrial surfaces in vivo. Cultures on rat-tail collagen were maintained for several weeks. These methods provide a valuable system for studying the endometrium in vitro.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalCell and Tissue Research
Volume261
Issue number1
DOIs
StatePublished - Jul 1990
Externally publishedYes

Keywords

  • Cell culture
  • Collagen
  • Endometrium
  • Holstein cow
  • Lectins
  • Polarity

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

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