Effects of storage and thawing conditions on coagulation testing

R. C. Gosselin, K. Honeychurch, H. J. Kang, Denis M Dwyre

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Introduction: Current recommendations for coagulation testing storage and thawing are based on historical studies that were performed using unbuffered 3.8% sodium citrate. We sought to measure the effects of freezing and thawing conditions 3.2% buffered sodium citrate plasma samples that have been stored in vials with either snap or sealed screw tops, frozen in -70 °C freezer or dry ice and thawed either capped or uncapped. Methods: Shed blood samples were pooled and then aliquoted into four snap top and four screw tops vials. Half the vials were stored in a -70 °C freezer, and half on dry ice for at least 16 h. Afterwards, half the frozen samples were thawed in 37 °C waterbath capped, and other half were thawed capped. After thawing cycles, samples were tested for PT, activated partial thromboplastin time (APTT), fibrinogen, D-dimer, factor assays, von Willebrand factor activity, plasminogen, antithrombin, protein C and lupus anticoagulant. Results: Prothrombin time, APTT, factor X, and lupus anticoagulant testing were affected by all vials, freezing and thawing conditions, whereas fibrinogen, D-dimer, von Willebrand activity or protein C were not affected by any vial, freezing or storage condition. Conclusions: Storage vials, freezing and thawing condition affect coagulation testing, although these differences may not be clinically significant.

Original languageEnglish (US)
Pages (from-to)551-559
Number of pages9
JournalInternational Journal of Laboratory Hematology
Volume37
Issue number4
DOIs
StatePublished - 2015

    Fingerprint

Keywords

  • Coagulation
  • Laboratory practice

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Hematology

Cite this