Effects of salinity and transparent exopolymer particles on formation of aquatic aggregates and their association with norovirus

Kaitlyn T. Hanley, Stefan Wuertz, Alexander Schriewer, Uta Passow, Woutrina A Smith, Paul Olin, Karen Shapiro

Research output: Contribution to journalArticle

Abstract

Human noroviruses (NoVs) are responsible for 50% of food-related disease outbreaks and are notably associated with shellfish consumption. Despite the detrimental health impacts of human NoV-contaminated seafood to public health, there is a lack of knowledge on the physicochemical conditions that govern NoV transmission in aquatic ecosystems. In the present study, we investigated the propensity for NoVs to associate with aquatic aggregates, which have been shown to efficiently deliver nano-sized particles to shellfish. Specific physicochemical conditions characteristic of shellfish cultivation waters, specifically salinity and transparent exopolymer particles (TEP), were targeted in this study for investigating aggregate formation and NoV association dynamics. Murine norovirus (MNV) was used in aggregation experiments as a model surrogate for NoVs. Results demonstrate increased aggregate formation as a function of increasing salinity and TEP concentrations, as well as greater numbers of MNV genomes incorporated into aggregates under conditions that favor aggregation. As aggregate formation was enhanced in waters representing optimal conditions for shellfish production, specifically saline and high TEP waters, the implications to virus transport and shellfish food safety are profound: more aggregates implies increased scavenging of virus particles from surrounding waters and therefor greater risk for bivalve contamination with nano-sized pathogens. These novel data provide insight into where and when NoVs are most likely to be ingested by shellfish via contaminated aggregates, thereby informing best management and water quality monitoring practices aimed at providing safe seafood to consumers.

Original languageEnglish (US)
Pages (from-to)1514-1521
Number of pages8
JournalScience of the Total Environment
Volume643
DOIs
StatePublished - Dec 1 2018

Fingerprint

transparent exopolymer particle
Shellfish
shellfish
salinity
Water
seafood
Viruses
Agglomeration
Food safety
virus particle
Aquatic ecosystems
food safety
Scavenging
health impact
Public health
Pathogens
effect
aquatic ecosystem
water
Water quality

Keywords

  • Pathogen
  • Seafood safety
  • Shellfish
  • Transmission
  • Transport
  • Water quality

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Effects of salinity and transparent exopolymer particles on formation of aquatic aggregates and their association with norovirus. / Hanley, Kaitlyn T.; Wuertz, Stefan; Schriewer, Alexander; Passow, Uta; Smith, Woutrina A; Olin, Paul; Shapiro, Karen.

In: Science of the Total Environment, Vol. 643, 01.12.2018, p. 1514-1521.

Research output: Contribution to journalArticle

@article{55d8bff29f45498e9c80e89f4cefe476,
title = "Effects of salinity and transparent exopolymer particles on formation of aquatic aggregates and their association with norovirus",
abstract = "Human noroviruses (NoVs) are responsible for 50{\%} of food-related disease outbreaks and are notably associated with shellfish consumption. Despite the detrimental health impacts of human NoV-contaminated seafood to public health, there is a lack of knowledge on the physicochemical conditions that govern NoV transmission in aquatic ecosystems. In the present study, we investigated the propensity for NoVs to associate with aquatic aggregates, which have been shown to efficiently deliver nano-sized particles to shellfish. Specific physicochemical conditions characteristic of shellfish cultivation waters, specifically salinity and transparent exopolymer particles (TEP), were targeted in this study for investigating aggregate formation and NoV association dynamics. Murine norovirus (MNV) was used in aggregation experiments as a model surrogate for NoVs. Results demonstrate increased aggregate formation as a function of increasing salinity and TEP concentrations, as well as greater numbers of MNV genomes incorporated into aggregates under conditions that favor aggregation. As aggregate formation was enhanced in waters representing optimal conditions for shellfish production, specifically saline and high TEP waters, the implications to virus transport and shellfish food safety are profound: more aggregates implies increased scavenging of virus particles from surrounding waters and therefor greater risk for bivalve contamination with nano-sized pathogens. These novel data provide insight into where and when NoVs are most likely to be ingested by shellfish via contaminated aggregates, thereby informing best management and water quality monitoring practices aimed at providing safe seafood to consumers.",
keywords = "Pathogen, Seafood safety, Shellfish, Transmission, Transport, Water quality",
author = "Hanley, {Kaitlyn T.} and Stefan Wuertz and Alexander Schriewer and Uta Passow and Smith, {Woutrina A} and Paul Olin and Karen Shapiro",
year = "2018",
month = "12",
day = "1",
doi = "10.1016/j.scitotenv.2018.06.300",
language = "English (US)",
volume = "643",
pages = "1514--1521",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effects of salinity and transparent exopolymer particles on formation of aquatic aggregates and their association with norovirus

AU - Hanley, Kaitlyn T.

AU - Wuertz, Stefan

AU - Schriewer, Alexander

AU - Passow, Uta

AU - Smith, Woutrina A

AU - Olin, Paul

AU - Shapiro, Karen

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Human noroviruses (NoVs) are responsible for 50% of food-related disease outbreaks and are notably associated with shellfish consumption. Despite the detrimental health impacts of human NoV-contaminated seafood to public health, there is a lack of knowledge on the physicochemical conditions that govern NoV transmission in aquatic ecosystems. In the present study, we investigated the propensity for NoVs to associate with aquatic aggregates, which have been shown to efficiently deliver nano-sized particles to shellfish. Specific physicochemical conditions characteristic of shellfish cultivation waters, specifically salinity and transparent exopolymer particles (TEP), were targeted in this study for investigating aggregate formation and NoV association dynamics. Murine norovirus (MNV) was used in aggregation experiments as a model surrogate for NoVs. Results demonstrate increased aggregate formation as a function of increasing salinity and TEP concentrations, as well as greater numbers of MNV genomes incorporated into aggregates under conditions that favor aggregation. As aggregate formation was enhanced in waters representing optimal conditions for shellfish production, specifically saline and high TEP waters, the implications to virus transport and shellfish food safety are profound: more aggregates implies increased scavenging of virus particles from surrounding waters and therefor greater risk for bivalve contamination with nano-sized pathogens. These novel data provide insight into where and when NoVs are most likely to be ingested by shellfish via contaminated aggregates, thereby informing best management and water quality monitoring practices aimed at providing safe seafood to consumers.

AB - Human noroviruses (NoVs) are responsible for 50% of food-related disease outbreaks and are notably associated with shellfish consumption. Despite the detrimental health impacts of human NoV-contaminated seafood to public health, there is a lack of knowledge on the physicochemical conditions that govern NoV transmission in aquatic ecosystems. In the present study, we investigated the propensity for NoVs to associate with aquatic aggregates, which have been shown to efficiently deliver nano-sized particles to shellfish. Specific physicochemical conditions characteristic of shellfish cultivation waters, specifically salinity and transparent exopolymer particles (TEP), were targeted in this study for investigating aggregate formation and NoV association dynamics. Murine norovirus (MNV) was used in aggregation experiments as a model surrogate for NoVs. Results demonstrate increased aggregate formation as a function of increasing salinity and TEP concentrations, as well as greater numbers of MNV genomes incorporated into aggregates under conditions that favor aggregation. As aggregate formation was enhanced in waters representing optimal conditions for shellfish production, specifically saline and high TEP waters, the implications to virus transport and shellfish food safety are profound: more aggregates implies increased scavenging of virus particles from surrounding waters and therefor greater risk for bivalve contamination with nano-sized pathogens. These novel data provide insight into where and when NoVs are most likely to be ingested by shellfish via contaminated aggregates, thereby informing best management and water quality monitoring practices aimed at providing safe seafood to consumers.

KW - Pathogen

KW - Seafood safety

KW - Shellfish

KW - Transmission

KW - Transport

KW - Water quality

UR - http://www.scopus.com/inward/record.url?scp=85049323148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049323148&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2018.06.300

DO - 10.1016/j.scitotenv.2018.06.300

M3 - Article

VL - 643

SP - 1514

EP - 1521

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -