Effects of roasting, blanching, autoclaving, and microwave heating on antigenicity of almond (Prunus dulcis L.) proteins

M. Venkatachalam, Suzanne S Teuber, K. H. Roux, S. K. Sathe

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Whole, unprocessed Nonpareil almonds were subjected to a variety of heat processing methods that included roasting (280, 300, and 320 °F for 20 and 30 min each; and 335 and 350 °F for 8, 10, and 12 min each), autoclaving (121 °C, 15 psi, for 5, 10, 15, 20, 25, and 30 min), blanching (100 °C for 1, 2, 3, 4, 5, and 10 min), and microwave heating (1, 2, and 3 min). Proteins were extracted from defatted almond flour in borate saline buffer, and immunoreactivity of the soluble proteins (normalized to 1 mg protein/mL for all samples) was determined using enzyme linked immunosorbent assay (ELISA). Antigenic stability of the almond major protein (amandin) in the heat-processed samples was determined by competitive inhibition ELISA using rabbit polyclonal antibodies raised against amandin. Processed samples were also assessed for heat stability of total antigenic proteins by sandwich ELISA using goat and rabbit polyclonal antibodies raised against unprocessed Nonpareil almond total protein extract. ELISA assays and Western blotting experiments that used both rabbit polyclonal antibodies and human IgE from pooled sera indicated antigenic stability of almond proteins when compared with that of the unprocessed counterpart.

Original languageEnglish (US)
Pages (from-to)3544-3548
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number12
DOIs
StatePublished - Jun 5 2002

Keywords

  • Almond
  • Amandin
  • Antigenicity
  • ELISA
  • Food allergy
  • Processing
  • Protein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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