Effects of resveratrol on growth and function of rat ovarian granulosa cells

Israel Ortega, Donna H. Wong, Jesus A. Villanueva, Amanda B. Cress, Anna Sokalska, Scott D Stanley, Antoni J. Duleba

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Objective: To evaluate the effects of resveratrol on growth and function of granulosa cells. Previously, we demonstrated that resveratrol exerts profound proapoptotic effects on theca-interstitial cells. Design: In vitro study. Setting: Research laboratory. Animal(s): Immature Sprague-Dawley female rats. Intervention(s): Granulosa cells were cultured in the absence or presence of resveratrol. Main Outcome Measure(s): DNA synthesis was determined by thymidine incorporation assay, apoptosis by activity of caspases 3/7, cell morphology by immunocytochemistry, steroidogenesis by mass spectrometry, antimüllerian hormone (AMH), and vascular endothelial growth factor (VEGF) expression by polymerase chain reaction and Western blot. Result(s): Resveratrol induced a biphasic effect on DNA synthesis, whereby a lower concentration stimulated thymidine incorporation and higher concentrations inhibited it. Additionally, resveratrol slightly increased the cell number and modestly decreased the activity of caspases 3/7 with no effect on cell morphology or progesterone production. However, resveratrol decreased aromatization and VEGF expression, whereas AMH expression remained unaltered. Conclusion(s): Resveratrol, by exerting cytostatic but not cytotoxic effects, together with antiangiogenic actions mediated by decreased VEGF in granulosa cells, may alter the ratio of theca-to-granulosa cells and decrease vascular permeability, and therefore may be of potential therapeutic use in conditions associated with highly vascularized theca-interstitial hyperplasia and abnormal angiogenesis, such as those seen in women with polycystic ovary syndrome.

Original languageEnglish (US)
Pages (from-to)1563-1573
Number of pages11
JournalFertility and Sterility
Issue number6
StatePublished - Dec 2012


  • AMH
  • Apoptosis
  • granulosa cells
  • proliferation
  • resveratrol
  • steroidogenesis
  • VEGF

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine


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