Effects of resveratrol on growth and function of rat ovarian granulosa cells

Israel Ortega, Donna H. Wong, Jesus A. Villanueva, Amanda B. Cress, Anna Sokalska, Scott D Stanley, Antoni J. Duleba

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: To evaluate the effects of resveratrol on growth and function of granulosa cells. Previously, we demonstrated that resveratrol exerts profound proapoptotic effects on theca-interstitial cells. Design: In vitro study. Setting: Research laboratory. Animal(s): Immature Sprague-Dawley female rats. Intervention(s): Granulosa cells were cultured in the absence or presence of resveratrol. Main Outcome Measure(s): DNA synthesis was determined by thymidine incorporation assay, apoptosis by activity of caspases 3/7, cell morphology by immunocytochemistry, steroidogenesis by mass spectrometry, antimüllerian hormone (AMH), and vascular endothelial growth factor (VEGF) expression by polymerase chain reaction and Western blot. Result(s): Resveratrol induced a biphasic effect on DNA synthesis, whereby a lower concentration stimulated thymidine incorporation and higher concentrations inhibited it. Additionally, resveratrol slightly increased the cell number and modestly decreased the activity of caspases 3/7 with no effect on cell morphology or progesterone production. However, resveratrol decreased aromatization and VEGF expression, whereas AMH expression remained unaltered. Conclusion(s): Resveratrol, by exerting cytostatic but not cytotoxic effects, together with antiangiogenic actions mediated by decreased VEGF in granulosa cells, may alter the ratio of theca-to-granulosa cells and decrease vascular permeability, and therefore may be of potential therapeutic use in conditions associated with highly vascularized theca-interstitial hyperplasia and abnormal angiogenesis, such as those seen in women with polycystic ovary syndrome.

Original languageEnglish (US)
Pages (from-to)1563-1573
Number of pages11
JournalFertility and Sterility
Volume98
Issue number6
DOIs
StatePublished - Dec 2012

Fingerprint

Granulosa Cells
Growth
Vascular Endothelial Growth Factor A
Caspase 7
Caspase 3
Thymidine
Hormones
Theca Cells
Polycystic Ovary Syndrome
DNA
Capillary Permeability
Cytostatic Agents
Therapeutic Uses
resveratrol
Hyperplasia
Progesterone
Sprague Dawley Rats
Mass Spectrometry
Cell Count
Western Blotting

Keywords

  • AMH
  • Apoptosis
  • granulosa cells
  • proliferation
  • resveratrol
  • steroidogenesis
  • VEGF

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Ortega, I., Wong, D. H., Villanueva, J. A., Cress, A. B., Sokalska, A., Stanley, S. D., & Duleba, A. J. (2012). Effects of resveratrol on growth and function of rat ovarian granulosa cells. Fertility and Sterility, 98(6), 1563-1573. https://doi.org/10.1016/j.fertnstert.2012.08.004

Effects of resveratrol on growth and function of rat ovarian granulosa cells. / Ortega, Israel; Wong, Donna H.; Villanueva, Jesus A.; Cress, Amanda B.; Sokalska, Anna; Stanley, Scott D; Duleba, Antoni J.

In: Fertility and Sterility, Vol. 98, No. 6, 12.2012, p. 1563-1573.

Research output: Contribution to journalArticle

Ortega, I, Wong, DH, Villanueva, JA, Cress, AB, Sokalska, A, Stanley, SD & Duleba, AJ 2012, 'Effects of resveratrol on growth and function of rat ovarian granulosa cells', Fertility and Sterility, vol. 98, no. 6, pp. 1563-1573. https://doi.org/10.1016/j.fertnstert.2012.08.004
Ortega, Israel ; Wong, Donna H. ; Villanueva, Jesus A. ; Cress, Amanda B. ; Sokalska, Anna ; Stanley, Scott D ; Duleba, Antoni J. / Effects of resveratrol on growth and function of rat ovarian granulosa cells. In: Fertility and Sterility. 2012 ; Vol. 98, No. 6. pp. 1563-1573.
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