Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors

Keith M. Stantz, Ning Cao, Bo Liu, Minsong Cao, Helen Chin-Sinex, Marc Mendonca, Jian-Jian Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Purpose: The purpose of this study is to monitor in vivo the IR dose dependent response of NF-κB and tumor hemodynamics as a function of time. Material and Methods: An MDA-231 breast cancer cell line was stably transfected with a firefly luciferase gene within the NF-kappaB promoter. Tumors on the right flank irradiated with a single fractionated dose of 5Gy or 10Gy. Over two weeks, photoacoustic spectroscopy (PCT-S), bioluminescence imaging (BLI), and dynamic contrast enhanced CT (DCE-CT) was used to monitor hemoglobin status, NF-kappaB expression, and physiology, respectively. Results: From the BLI, an increase in NF-kappaB expression was observed in both the right (irradiation) and left (nonirradiated) tumors, which peaked at 8-12 hours, returned to basal levels after 24 hours, and increased a second time from 3 to 7 days. This data identifies both a radiation-induced bystander effect and a bimodal longitudinal response associated with NF-κB-controlled luciferase promoter. The physiological results from DCE-CT measured an increase in perfusion (26%) two days after radiation and both a decrease in perfusion and an increase in fp by week 1 (10Gy cohort). PCT-S measured increased levels of oxygen saturation two days post IR, which did not change after 1 week. Initially, NF-κB would modify hemodynamics to increase oxygen delivery after IR insult. The secondary response appears to modulate tumor angiogenesis. Conclusions: A bimodal response to radiation was detected with NF-kappaB-controlled luciferase reporter with a concomitant hemodynamic response associated with tumor hypoxia. Experiments are being performed to increase statistics.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
DOIs
StatePublished - 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2010
CountryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

Fingerprint

hemodynamics
NF-kappa B
Radiation Effects
Hemodynamics
breast
Tumors
tumors
Breast Neoplasms
Radiation
Bioluminescence
Photoacoustic spectroscopy
bioluminescence
radiation
Luciferases
photoacoustic spectroscopy
Neoplasms
Spectrum Analysis
Perfusion
Oxygen
Bystander Effect

Keywords

  • Breast cancer
  • DCE-CT
  • Ionizing radiation
  • NF-kappaB
  • Oxygen saturation
  • Photoacoustic computed tomographic spectroscopy
  • Physiology

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Stantz, K. M., Cao, N., Liu, B., Cao, M., Chin-Sinex, H., Mendonca, M., & Li, J-J. (2010). Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7564). [75641J] https://doi.org/10.1117/12.842238

Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. / Stantz, Keith M.; Cao, Ning; Liu, Bo; Cao, Minsong; Chin-Sinex, Helen; Mendonca, Marc; Li, Jian-Jian.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564 2010. 75641J.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stantz, KM, Cao, N, Liu, B, Cao, M, Chin-Sinex, H, Mendonca, M & Li, J-J 2010, Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7564, 75641J, Photons Plus Ultrasound: Imaging and Sensing 2010, San Francisco, CA, United States, 1/24/10. https://doi.org/10.1117/12.842238
Stantz KM, Cao N, Liu B, Cao M, Chin-Sinex H, Mendonca M et al. Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564. 2010. 75641J https://doi.org/10.1117/12.842238
Stantz, Keith M. ; Cao, Ning ; Liu, Bo ; Cao, Minsong ; Chin-Sinex, Helen ; Mendonca, Marc ; Li, Jian-Jian. / Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564 2010.
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