Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)

Wilson F. Ramírez-Duarte, Jiali Jin, Tomofumi Kurobe, Swee J Teh

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.

Original languageEnglish (US)
Pages (from-to)26-32
Number of pages7
JournalScience of the Total Environment
Volume568
DOIs
StatePublished - Oct 15 2016

Keywords

  • Acidification
  • Fish
  • Glutathione peroxidase
  • Oxidative stress
  • RT-qPCR
  • Soft water

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

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