Abstract
Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 26-32 |
| Number of pages | 7 |
| Journal | Science of the Total Environment |
| Volume | 568 |
| DOIs | |
| State | Published - Oct 15 2016 |
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Keywords
- Acidification
- Fish
- Glutathione peroxidase
- Oxidative stress
- RT-qPCR
- Soft water
ASJC Scopus subject areas
- Environmental Chemistry
- Pollution
- Waste Management and Disposal
- Environmental Engineering
Cite this
Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes). / Ramírez-Duarte, Wilson F.; Jin, Jiali; Kurobe, Tomofumi; Teh, Swee J.
In: Science of the Total Environment, Vol. 568, 15.10.2016, p. 26-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)
AU - Ramírez-Duarte, Wilson F.
AU - Jin, Jiali
AU - Kurobe, Tomofumi
AU - Teh, Swee J
PY - 2016/10/15
Y1 - 2016/10/15
N2 - Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.
AB - Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.
KW - Acidification
KW - Fish
KW - Glutathione peroxidase
KW - Oxidative stress
KW - RT-qPCR
KW - Soft water
UR - http://www.scopus.com/inward/record.url?scp=84973400131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973400131&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2016.05.179
DO - 10.1016/j.scitotenv.2016.05.179
M3 - Article
C2 - 27285793
AN - SCOPUS:84973400131
VL - 568
SP - 26
EP - 32
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
ER -