Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)

Wilson F. Ramírez-Duarte, Jiali Jin, Tomofumi Kurobe, Swee J Teh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.

Original languageEnglish (US)
Pages (from-to)26-32
Number of pages7
JournalScience of the Total Environment
Volume568
DOIs
StatePublished - Oct 15 2016

Fingerprint

Antioxidants
antioxidant
Fish
Aquatic ecosystems
Thiobarbituric Acid Reactive Substances
Acidification
fish
Glutathione Peroxidase
aquatic ecosystem
Lipids
acidification
Glutathione
Toxicity
Reactive Oxygen Species
lipid
toxicity
Oxygen
Acids
Water
acid

Keywords

  • Acidification
  • Fish
  • Glutathione peroxidase
  • Oxidative stress
  • RT-qPCR
  • Soft water

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

Cite this

Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes). / Ramírez-Duarte, Wilson F.; Jin, Jiali; Kurobe, Tomofumi; Teh, Swee J.

In: Science of the Total Environment, Vol. 568, 15.10.2016, p. 26-32.

Research output: Contribution to journalArticle

@article{498e3b71eacc4441a5a61defb5fe9859,
title = "Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)",
abstract = "Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.",
keywords = "Acidification, Fish, Glutathione peroxidase, Oxidative stress, RT-qPCR, Soft water",
author = "Ram{\'i}rez-Duarte, {Wilson F.} and Jiali Jin and Tomofumi Kurobe and Teh, {Swee J}",
year = "2016",
month = "10",
day = "15",
doi = "10.1016/j.scitotenv.2016.05.179",
language = "English (US)",
volume = "568",
pages = "26--32",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effects of prolonged exposure to low pH on enzymatic and non-enzymatic antioxidants in Japanese Medaka (Oryzias latipes)

AU - Ramírez-Duarte, Wilson F.

AU - Jin, Jiali

AU - Kurobe, Tomofumi

AU - Teh, Swee J

PY - 2016/10/15

Y1 - 2016/10/15

N2 - Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.

AB - Acidification in aquatic ecosystems is a major concern worldwide. In freshwater, although there are several publications reporting acute toxicity and adverse effects due to low pH, little is known about adverse effects on antioxidant mechanisms in fish after prolonged exposure. This study aimed to investigate how antioxidants are affected by raising larval Japanese Medaka (Oryzias latipes) in soft water at pH 5.5, 6.0, and 6.5. After 18 days of exposure, glutathione concentration and glutathione peroxidase activity in whole body homogenates increased as pH decreased, without changes in lipid peroxidation measured as thiobarbituric acid reactive substances. This study showed that prolonged exposure to low pH increased reactive oxygen species production and that fish cope with it by increasing levels of enzymatic and non-enzymatic antioxidants.

KW - Acidification

KW - Fish

KW - Glutathione peroxidase

KW - Oxidative stress

KW - RT-qPCR

KW - Soft water

UR - http://www.scopus.com/inward/record.url?scp=84973400131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973400131&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2016.05.179

DO - 10.1016/j.scitotenv.2016.05.179

M3 - Article

C2 - 27285793

AN - SCOPUS:84973400131

VL - 568

SP - 26

EP - 32

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -