Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma

Jamie A. Textor, Jeffrey W. Norris, Fern Tablin

Research output: Contribution to journalArticle

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Abstract

Objective - To test the hypotheses that preparation method, exposure to shear force, and exposure to collagen affect the release of growth factors from equine platelet-rich plasma (PRP). Sample Population - PRP obtained from 6 horses. Procedures - PRP was prepared via 2 preparation methods (tube and automated) and subjected to 6 treatment conditions (resting, detergent, exposure to shear via 21- and 25-gauge needles, and exposure to collagen [10 and 20 μ/mL]). Concentrations of platelet-derived growth factor, isoform BB (PDGF-BB); transforming growth factor β, isoform 1 (TGFβ1); and insulin-like growth factor, isoform 1 (IGF-1) were quantified by use of ELISAs. Statistical analysis was conducted via repeated-measures ANOVA. Results - Platelet numbers were significantly higher in tube-prepared PRP than in automatedprepared PRP. Growth factor concentrations did not differ significantly between preparation methods. Mean PDGF-BB concentration ranged from 134 to 7,157 pg/mL, mean TGF̀1 concentration ranged from 1,153 to 22,677 pg/mL, and mean IGF-1 concentration ranged from 150 to 280 ng/mL. Shear force did not affect growth factor concentrations. Dosedependent increases in PDGF-BB and TGFβ1 were detected in response to collagen, but equalled only 10% of the estimated total platelet content. Concentrations of IGF-1 were not significantly different among treatments and negative or positive control treatments. Serum concentrations of PDGF-BB and TGFβ1 exceeded concentrations in PRP for most treatment conditions. Conclusions and Clinical Relevance-Release of growth factors from equine PRP was negligible as a result of the injection process alone. Investigation of platelet-activation protocols is warranted to potentially enhance PRP treatment efficacy in horses.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume72
Issue number2
DOIs
StatePublished - Feb 2011

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platelet-derived growth factor
transforming growth factors
Platelet-Rich Plasma
growth factors
Horses
shears
collagen
Intercellular Signaling Peptides and Proteins
Protein Isoforms
somatomedins
Collagen
horses
Transforming Growth Factors
platelet activation
Somatomedins
blood platelet count
gauges
methodology
detergents
statistical analysis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma. / Textor, Jamie A.; Norris, Jeffrey W.; Tablin, Fern.

In: American Journal of Veterinary Research, Vol. 72, No. 2, 02.2011, p. 271-278.

Research output: Contribution to journalArticle

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