Effects of positive end-expiratory pressure and recruitment maneuvers in a ventilator-induced injury mouse model

Laura A. Cagle, Lisa M. Franzi, Angela L. Linderholm, Jerold A Last, Jason Yeates Adams, Richart W Harper, Nicholas Kenyon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background Positive-pressure mechanical ventilation is an essential therapeutic intervention, yet it causes the clinical syndrome known as ventilator-induced lung injury. Various lung protective mechanical ventilation strategies have attempted to reduce or prevent ventilator-induced lung injury but few modalities have proven effective. A model that isolates the contribution of mechanical ventilation on the development of acute lung injury is needed to better understand biologic mechanisms that lead to ventilator-induced lung injury. Objectives To evaluate the effects of positive end-expiratory pressure and recruitment maneuvers in reducing lung injury in a ventilator-induced lung injury murine model in short- and longer-term ventilation. Methods 5–12 week-old female BALB/c mice (n = 85) were anesthetized, placed on mechanical ventilation for either 2 hrs or 4 hrs with either low tidal volume (8 ml/kg) or high tidal volume (15 ml/kg) with or without positive end-expiratory pressure and recruitment maneuvers. Results Alteration of the alveolar-capillary barrier was noted at 2 hrs of high tidal volume ventilation. Standardized histology scores, influx of bronchoalveolar lavage albumin, proinflammatory cytokines, and absolute neutrophils were significantly higher in the high-tidal volume ventilation group at 4 hours of ventilation. Application of positive end-expiratory pressure resulted in significantly decreased standardized histology scores and bronchoalveolar absolute neutrophil counts at low- and high-tidal volume ventilation, respectively. Recruitment maneuvers were essential to maintain pulmonary compliance at both 2 and 4 hrs of ventilation.

Original languageEnglish (US)
Article numbere0187419
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

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ventilators
Ventilator-Induced Lung Injury
Positive-Pressure Respiration
Tidal Volume
Mechanical Ventilators
Ventilation
Artificial Respiration
tidal volume
animal models
lungs
Wounds and Injuries
Histology
Neutrophils
Lung Compliance
Acute Lung Injury
histology
neutrophils
Lung Injury
Bronchoalveolar Lavage
Albumins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Effects of positive end-expiratory pressure and recruitment maneuvers in a ventilator-induced injury mouse model. / Cagle, Laura A.; Franzi, Lisa M.; Linderholm, Angela L.; Last, Jerold A; Adams, Jason Yeates; Harper, Richart W; Kenyon, Nicholas.

In: PLoS One, Vol. 12, No. 11, e0187419, 01.11.2017.

Research output: Contribution to journalArticle

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