Effects of physiological electric fields on migration of human dermal fibroblasts

Aihua Guo, Bing Song, Brian Reid, Yu Gu, John V. Forrester, Colin A B Jahoda, Min Zhao

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Endogenous electric currents generated instantly at skin wounds direct migration of epithelial cells and are likely to be important in wound healing. Migration of fibroblasts is critical in wound healing. It remains unclear how wound electric fields guide migration of dermal fibroblasts. We report here that mouse skin wounds generated endogenous electric currents for many hours. Human dermal fibroblasts of both primary and cell-line cultures migrated directionally but slowly toward the anode in an electric field of 50-100 mV mm-1. This is different from keratinocytes, which migrate quickly to the cathode. It took more than 1 hour for dermal fibroblasts to manifest detectable directional migration. Larger field strength (400 mV mm 1) was required to induce directional migration within 1 hour after onset of the field. Phosphatidylinositol-3-OH kinase (PI3 kinase) mediates cathode-directed migration of keratinocytes. We tested the role of PI3 kinase in anode-directed migration of fibroblasts. An applied electric field activated PI3 kinase/Akt in dermal fibroblasts. Dermal fibroblasts from p110γ (a PI3 kinase catalytic subunit) null mice showed significantly decreased directional migration. These results suggest that physiological electric fields may regulate motility of dermal fibroblasts and keratinocytes differently, albeit using similar PI3 kinase-dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)2320-2327
Number of pages8
JournalJournal of Investigative Dermatology
Volume130
Issue number9
DOIs
StatePublished - Sep 2010

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Fibroblasts
Electric fields
Phosphatidylinositol 3-Kinases
Skin
Electrodes
Keratinocytes
Electric currents
Wound Healing
Wounds and Injuries
Anodes
Cathodes
Primary Cell Culture
Cell culture
Catalytic Domain
Epithelial Cells
Cell Line

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Effects of physiological electric fields on migration of human dermal fibroblasts. / Guo, Aihua; Song, Bing; Reid, Brian; Gu, Yu; Forrester, John V.; Jahoda, Colin A B; Zhao, Min.

In: Journal of Investigative Dermatology, Vol. 130, No. 9, 09.2010, p. 2320-2327.

Research output: Contribution to journalArticle

Guo, Aihua ; Song, Bing ; Reid, Brian ; Gu, Yu ; Forrester, John V. ; Jahoda, Colin A B ; Zhao, Min. / Effects of physiological electric fields on migration of human dermal fibroblasts. In: Journal of Investigative Dermatology. 2010 ; Vol. 130, No. 9. pp. 2320-2327.
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