Effects of multiple chondroitinase ABC applications on tissue engineered articular cartilage

Roman M. Natoli, Donald J. Responte, Benjamin Y. Lu, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Increasing tensile properties and collagen content is a recognized need in articular cartilage tissue engineering. This study tested the hypothesis that multiple applications of chondroitinase ABC (C-ABC), a glycosaminoglycan (GAG) degrading enzyme, could increase construct tensile properties ina scaffold-less approach for articular cartilage tissue engineering. Developing constructs were treated with C-ABC at 2 weeks, 4 weeks, or both 2 and 4 weeks. At 4 and 6 weeks, construct sulfated GAG composition, collagen composition, and compressive and tensile biomechanical properties were assessed, along with immunohistochemistry (IHC) for collagens type I, II, and VI, and the proteoglycan decorin. At 6 weeks, the tensile modulus and ultimate tensile strength of the group treated at both 2 and 4 weeks were significantly increased over controls by 78% and 64%, reaching values of 3.4 and 1.4 MPa, respectively. Collagen concentration also increased 43%. Further, groups treated at either 2 weeks or 4 weeks alone also had increased tensile stiffness compared to controls. Surprisingly, though GAG was depleted in the treated groups, by 6 weeks there were no significant differences in compressive stiffness. IHC showed abundant collagen type II and VI in all groups, with no collagen type I. Further, decorin staining was reduced following C-ABC treatment, but returned during subsequent culture. The results support the use of C-ABC in cartilage tissue engineering for increasing tensile properties.

Original languageEnglish (US)
Pages (from-to)949-956
Number of pages8
JournalJournal of Orthopaedic Research
Volume27
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

Fingerprint

Chondroitin ABC Lyase
Articular Cartilage
Tissue Engineering
Collagen Type VI
Decorin
Collagen
Collagen Type II
A73025
Collagen Type I
Glycosaminoglycans
Immunohistochemistry
Tensile Strength
Proteoglycans
Cartilage
Staining and Labeling
Enzymes

Keywords

  • Biomechanics
  • Collagen
  • Glycosaminoglycans
  • Mechanobiology
  • Tensile properties

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Effects of multiple chondroitinase ABC applications on tissue engineered articular cartilage. / Natoli, Roman M.; Responte, Donald J.; Lu, Benjamin Y.; Athanasiou, Kyriacos A.

In: Journal of Orthopaedic Research, Vol. 27, No. 7, 07.2009, p. 949-956.

Research output: Contribution to journalArticle

Natoli, Roman M. ; Responte, Donald J. ; Lu, Benjamin Y. ; Athanasiou, Kyriacos A. / Effects of multiple chondroitinase ABC applications on tissue engineered articular cartilage. In: Journal of Orthopaedic Research. 2009 ; Vol. 27, No. 7. pp. 949-956.
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