Effects of maternal exposure to estrogen and PCB on different life stages of zebrafish (Danio rerio)

Per Erik Olsson, Liselotte Westerlund, Swee J Teh, Karin Billsson, A. Håkan Berg, Mats Tysklind, Jan Nilsson, Lars Ove Eriksson, David E. Hinton

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

PCBs have been found to impair both reproduction and development in fish. We have investigated the effects of 3 PCB congeners, 2,3,3',4,4',5,6-HpCB (PCB-190); 2,3,4,4'-TeCB (PCB-60); and 2,2',4,6,6'-PeCB (PCB-104), and the estrogenic hormone 17β-estradiol on fecundity, early life-stage mortality, gross morphology and histology of zebrafish (Danio redo). While none of the studied substances reduced fecundity, they increased embryo and larval mortality. The most severe effects on viability were observed following treatment with 17β-estradiol or the weakly estrogenic PCB-104. Following 17β-estradiol or PCB-104 exposure, mortality continued through the yolksac absorption phase. PCB-60, on the other hand, resulted in mortality between the 30% epiboly stage and 75% epiboly stage. At the same time as embryos started to die, embryo development and hatching were delayed. PCB-190 showed only moderate effects on early-life stage mortality. The fish were reared until sexual maturation whereafter they were subjected to gross morphological and histological analyses. Changes in morphology were observed following PCB-104 and PCB-190 treatment. Both substances gave rise to craniofacial malformations while PCB-104 also led to lordosis in females and scoliosis in fish of both sexes. From histological analysis it was found that PCB-104 and 17β-estradiol resulted in karyorrhexis and karyolysis in the kidney. Possible signs of bile stasis were observed following 17β-estradiol and PCB-190 treatment. Some effects were observed on the gonads, including areas in the ovary showing atresia and limited failure of testicular spermatogenesis in 17β-estradiol, PCB-104, and PCB-60 treated fish. While all studied substances resulted in effects on offspring, the observation that estrogenic substances are highly embryotoxic, raises concern that endocrine disrupting substances may severely reduce fish populations in polluted areas.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalAmbio
Volume28
Issue number1
StatePublished - Feb 1999

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Environmental Engineering

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    Olsson, P. E., Westerlund, L., Teh, S. J., Billsson, K., Berg, A. H., Tysklind, M., Nilsson, J., Eriksson, L. O., & Hinton, D. E. (1999). Effects of maternal exposure to estrogen and PCB on different life stages of zebrafish (Danio rerio). Ambio, 28(1), 100-106.