Effects of 'loop' diuretics on ion transport by dog tracheal epithelium

Jonathan Widdicombe, I. T. Nathanson, E. Highland

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The 'loop' diuretics MK-196, bumetanide, piretanide, and furosemide are all potent inhibitors of Cl transport by the dog's tracheal epithelium. In short-circuited tissues, the drugs caused significant decreases in both unidirectional Cl fluxes and in the net flux of Cl toward the lumen; the change in net Cl flux was not significantly different from the change in short circuit current. The drugs had no effect on active Na absorption. All drugs caused a significant fall in tissue conductance. All drugs, except MK-196, were more potent from the serosal bath; MK-196 was equipotent from either side of the tissue. In experiments with isolated cells, the diuretics caused no significant changes in intracellular Na and K concentrations, a fall in intracellular Cl concentration, and approximately equal falls in Na and Cl influxes. These results suggest that the site of action of these drugs is on a basolateral linked Na-Cl entry process. Additional evidence for such a linked entry process was provided by experiments in which removal of Cl reduced Na influx and removal of Na reduced Cl influx.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Issue number3
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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