Effects of long-term endocrine disrupting compound exposure on Macaca mulatta embryonic stem cells

Uros Midic, Kailey A. Vincent, Catherine A. VandeVoort, Keith E. Latham

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Endocrine disrupting chemicals (EDCs) exert significant effects on health and physiology, many traceable to effects on stem cell programming underlying development. Understanding risk of low-level, chronic EDC exposure will be enhanced by knowledge of effects on stem cells. We exposed rhesus monkey embryonic stem cells to low levels of five EDCs [bisphenol A (BPA), atrazine (ATR), tributyltin (TBT), perfluorooctanoic acid (PFOA), and di-(2-ethylhexyl) phthalate (DEHP)] for 28 days, and evaluated effects on gene expression by RNAseq transcriptome profiling. We observed little effect of BPA, and small numbers of affected genes (≤119) with other EDCs. There was substantial overlap in effects across two, three, or four treatments. Ingenuity Pathway analysis indicated suppression of cell survival genes and genes downstream of several stress response mediators, activation of cell death genes, and modulations in several genes regulating pluripotency, differentiation, and germ layer development. Potential adverse effects of these changes on development are discussed.

Original languageEnglish (US)
Pages (from-to)382-393
Number of pages12
JournalReproductive Toxicology
Volume65
DOIs
StatePublished - Oct 1 2016

Keywords

  • Atrazine
  • Bisphenol a
  • Developmental origins of disease
  • Endocrine disruptor
  • Gene-environment interaction
  • Nuclear programming
  • Obesogen
  • Perfluorooctanoic acid
  • Phthalate
  • Transcriptome
  • Tributyltin

ASJC Scopus subject areas

  • Toxicology

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