Effects of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1

David J Maggs, B. Keith Collins, James G. Thorne, Mark P. Nasisse

Research output: Contribution to journalArticle

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Abstract

Objective - To determine the effects of varios concentrations of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1 (FHV-1). Sample Population - Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727. Procedure - Uninfected CRFK cells or CRFK cells infected with FHV-1 were cultured in Dulbecco's modified Eagle's medium or in 1 of 7 test media containing various concentrations of lysine and arginine. Viral titer and CRFK growth rate were assessed in each medium. Results - Media depleted of arginine almost completely inhibited viral replication, whereas 2.5 or 5.0 μg of arginine/ml of media was associated with a significant increase in FHV-1 replication. In media with 2.5 μg of arginine/ml, supplementation with 200 or 300 μg of lysine/ml reduced viral replication by 34.2 and 53.9%, respectively. This effect was not seen in media containing 5.0 μg of arginine/ml. Growth rates of CRFK cells also were suppressed in media containing these concentrations of amino acids, but they were not significantly different from each other. Conclusions and Clinical Relevance - Arginine exerts a substantial growth-promoting effect on FHV-1. Supplementation of viral culture medium with lysine attenuates this growth-promoting effect in media containing low concentrations of arginine. Analysis of data from this study indicates that high concentrations of lysine reduce in vitro replication of FHV-1 but only in media containing low concentrations of arginine. Clinical trials will be necessary to determine whether supplemental administration of lysine, with or without arginine restriction, will be useful in the management of cats with FHV-1 infections.

Original languageEnglish (US)
Pages (from-to)1474-1478
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume61
Issue number12
StatePublished - Dec 2000

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Felid herpesvirus 1
Herpesviridae
Felidae
Lysine
arginine
Arginine
lysine
cats
kidney cells
Kidney
virus replication
Growth
In Vitro Techniques
Herpesviridae Infections
eagles
Eagles
viral load
clinical trials
data analysis
culture media

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effects of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1. / Maggs, David J; Collins, B. Keith; Thorne, James G.; Nasisse, Mark P.

In: American Journal of Veterinary Research, Vol. 61, No. 12, 12.2000, p. 1474-1478.

Research output: Contribution to journalArticle

Maggs, David J ; Collins, B. Keith ; Thorne, James G. ; Nasisse, Mark P. / Effects of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1. In: American Journal of Veterinary Research. 2000 ; Vol. 61, No. 12. pp. 1474-1478.
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abstract = "Objective - To determine the effects of varios concentrations of L-lysine and L-arginine on in vitro replication of feline herpesvirus type-1 (FHV-1). Sample Population - Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727. Procedure - Uninfected CRFK cells or CRFK cells infected with FHV-1 were cultured in Dulbecco's modified Eagle's medium or in 1 of 7 test media containing various concentrations of lysine and arginine. Viral titer and CRFK growth rate were assessed in each medium. Results - Media depleted of arginine almost completely inhibited viral replication, whereas 2.5 or 5.0 μg of arginine/ml of media was associated with a significant increase in FHV-1 replication. In media with 2.5 μg of arginine/ml, supplementation with 200 or 300 μg of lysine/ml reduced viral replication by 34.2 and 53.9{\%}, respectively. This effect was not seen in media containing 5.0 μg of arginine/ml. Growth rates of CRFK cells also were suppressed in media containing these concentrations of amino acids, but they were not significantly different from each other. Conclusions and Clinical Relevance - Arginine exerts a substantial growth-promoting effect on FHV-1. Supplementation of viral culture medium with lysine attenuates this growth-promoting effect in media containing low concentrations of arginine. Analysis of data from this study indicates that high concentrations of lysine reduce in vitro replication of FHV-1 but only in media containing low concentrations of arginine. Clinical trials will be necessary to determine whether supplemental administration of lysine, with or without arginine restriction, will be useful in the management of cats with FHV-1 infections.",
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