Abstract
The presence of blood proteins and excess liquid in the airway lumen during airway inflammation may be secondary to extravasation and elevation of subepithelial hydrostatic pressure. This study examines how hydrostatic pressures of 5-20 cm H2O affect hydraulic conductivity and macromolecular permeability of primary cultures of bovine tracheal epithelium. Hydraulic conductivity was not altered by transepithelial pressure gradients of up to 20 cm H2O directed from the mucosal to serosal side of the tissue (m-s). By contrast, a 20-cm H2O s-m pressure resulted in a marked increase in hydraulic conductivity with the critical pressure lying between 10 and 20 cm H2O. Electrical conductance (i.e., permeability to ions) was not altered by m-s pressure gradients, or by a 5-cm H20 s-m gradient, but was increased by s-m pressures ≤ 10 cm. Fluxes (s-m and m-s) of fluorescein and fluorescent dextrans (70 and 2000 kDa) were not altered by pressures of up to 20 cm H2O m-s. By contrast s-m pressure gradients of 20 cm H2O dramatically increased the s-m fluxes of these probes. The increases in flux were completely reversible. The results indicate that s-m pressure gradients greatly increase the hydraulic conductivity of airway epithelium by creating pores with an effective diameter greater than 54 nm.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-267 |
| Number of pages | 11 |
| Journal | Experimental Lung Research |
| Volume | 23 |
| Issue number | 3 |
| State | Published - 1997 |
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Keywords
- Airway epithelium
- Airway inflammation
- Hydraulic conductivity
- Hydrostatic pressure
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Effects of hydrostatic pressure on permeability of airway epithelium. / Azizi, F.; Matsumoto, P. S.; Wu, D. X Y; Widdicombe, Jonathan.
In: Experimental Lung Research, Vol. 23, No. 3, 1997, p. 257-267.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of hydrostatic pressure on permeability of airway epithelium
AU - Azizi, F.
AU - Matsumoto, P. S.
AU - Wu, D. X Y
AU - Widdicombe, Jonathan
PY - 1997
Y1 - 1997
N2 - The presence of blood proteins and excess liquid in the airway lumen during airway inflammation may be secondary to extravasation and elevation of subepithelial hydrostatic pressure. This study examines how hydrostatic pressures of 5-20 cm H2O affect hydraulic conductivity and macromolecular permeability of primary cultures of bovine tracheal epithelium. Hydraulic conductivity was not altered by transepithelial pressure gradients of up to 20 cm H2O directed from the mucosal to serosal side of the tissue (m-s). By contrast, a 20-cm H2O s-m pressure resulted in a marked increase in hydraulic conductivity with the critical pressure lying between 10 and 20 cm H2O. Electrical conductance (i.e., permeability to ions) was not altered by m-s pressure gradients, or by a 5-cm H20 s-m gradient, but was increased by s-m pressures ≤ 10 cm. Fluxes (s-m and m-s) of fluorescein and fluorescent dextrans (70 and 2000 kDa) were not altered by pressures of up to 20 cm H2O m-s. By contrast s-m pressure gradients of 20 cm H2O dramatically increased the s-m fluxes of these probes. The increases in flux were completely reversible. The results indicate that s-m pressure gradients greatly increase the hydraulic conductivity of airway epithelium by creating pores with an effective diameter greater than 54 nm.
AB - The presence of blood proteins and excess liquid in the airway lumen during airway inflammation may be secondary to extravasation and elevation of subepithelial hydrostatic pressure. This study examines how hydrostatic pressures of 5-20 cm H2O affect hydraulic conductivity and macromolecular permeability of primary cultures of bovine tracheal epithelium. Hydraulic conductivity was not altered by transepithelial pressure gradients of up to 20 cm H2O directed from the mucosal to serosal side of the tissue (m-s). By contrast, a 20-cm H2O s-m pressure resulted in a marked increase in hydraulic conductivity with the critical pressure lying between 10 and 20 cm H2O. Electrical conductance (i.e., permeability to ions) was not altered by m-s pressure gradients, or by a 5-cm H20 s-m gradient, but was increased by s-m pressures ≤ 10 cm. Fluxes (s-m and m-s) of fluorescein and fluorescent dextrans (70 and 2000 kDa) were not altered by pressures of up to 20 cm H2O m-s. By contrast s-m pressure gradients of 20 cm H2O dramatically increased the s-m fluxes of these probes. The increases in flux were completely reversible. The results indicate that s-m pressure gradients greatly increase the hydraulic conductivity of airway epithelium by creating pores with an effective diameter greater than 54 nm.
KW - Airway epithelium
KW - Airway inflammation
KW - Hydraulic conductivity
KW - Hydrostatic pressure
UR - http://www.scopus.com/inward/record.url?scp=0030962681&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030962681&partnerID=8YFLogxK
M3 - Article
C2 - 9184792
AN - SCOPUS:0030962681
VL - 23
SP - 257
EP - 267
JO - Experimental Lung Research
JF - Experimental Lung Research
SN - 0190-2148
IS - 3
ER -