Effects of hydrostatic pressure on leporine meniscus cell-seeded PLLA scaffolds

Najmuddin J. Gunja, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Hydrostatic pressure (HP) is an important component of the loading environment of the knee joint. Studies with articular chondrocytes and TMJ disc fibrochondrocytes have identified certain benefits of HP for tissue engineering purposes. However, similar studies with meniscus cells are lacking. Thus, in this experiment, the effects of applying 10 MPa of HP at three different frequencies (0, 0.1, and 1 Hz) to leporine meniscus cell-seeded PLLA scaffolds were examined. HP was applied once every 3 days for 1 h for a period of 28 days. Constructs were analyzed for cellular, biochemical, and biomechanical properties. At t 5 4 weeks, total collagen/scaffold was found to be significantly higher in the 10 MPa, 0 Hz group when compared with other groups. This despite the fact that the cell numbers/scaffold were found to be lower in all HP groups when compared with the culture control. Additionally, the total GAG/scaffold, instantaneous modulus, and relaxation modulus were significantly increased in the 10 MPa, 0 Hz group when compared with the culture control. In summary, this experiment provides evidence for the benefit of a 10 MPa, 0 Hz stimulus, on both biochemical and biomechanical aspects, for the purposes of meniscus tissue engineering using PLLA scaffolds.

Original languageEnglish (US)
Pages (from-to)896-905
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume92
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Keywords

  • Hydrostatic pressure
  • Knee meniscus
  • PLLA
  • TGF-β1
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

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